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  • Featured Product
  • KD/KO Validated

COPS6 Polyclonal antibody, PBS Only

COPS6 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig, monkey

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10256-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COPS6 Polyclonal antibody (10256-1-AP)

    WB analysis using 10256-1-AP (same clone as 10256-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10256-1-AP (COPS6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10256-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of 3T3-L1 cells using COPS6 Polyclonal antibody (10256-1-AP)

    WB analysis of 3T3-L1 using 10256-1-AP (same clone as 10256-1-PBS)

    3T3-L1 cells were subjected to SDS PAGE followed by western blot with 10256-1-AP (COPS6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10256-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using COPS6 Polyclonal antibody (10256-1-AP)

    IHC staining of human colon using 10256-1-AP (same clone as 10256-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10256-1-AP (COPS6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10256-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using COPS6 Polyclonal antibody (10256-1-AP)

    IHC staining of human colon using 10256-1-AP (same clone as 10256-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10256-1-AP (COPS6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10256-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of COLO 320 cells using COPS6 Polyclonal antibody (10256-1-AP)

    IF Staining of COLO 320 using 10256-1-AP (same clone as 10256-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed COLO 320 cells using COPS6 antibody (10256-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10256-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using COPS6 Polyclonal antibody (10256-1-AP)

    IF Staining of HeLa using 10256-1-AP (same clone as 10256-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using COPS6 antibody (10256-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10256-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10256-1-PBS targets COPS6 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, monkey samples.

Tested Reactivity human, mouse, rat, pig, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COPS6 fusion protein Ag0331 相同性解析による交差性が予測される生物種
Full Name COP9 constitutive photomorphogenic homolog subunit 6 (Arabidopsis)
Calculated molecular weight 327 aa, 36 kDa
Observed molecular weight34 kDa
GenBank accession numberBC002520
Gene symbol COPS6
Gene ID (NCBI) 10980
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The COPS6 protein, also named mov34, is a 34 kDa subunit of COP9 signalosome, a highly conserved eight-subunit protein complex that functions as an important regulator in multiple signaling pathways (PMID: 38308184). This protein belongs to translation initiation factor 3 (eIF3) superfamily. It is involved in the regulation of cell cycle and acts as the regulatory subunit of the 26S proteasome.