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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COPS8 Recombinant antibody (83312-1-RR)

    WB analysis using 83312-1-RR (same clone as 83312-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83312-1-RR (COPS8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83312-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using COPS8 Recombinant antibody (83312-1-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83312-1-RR (same clone as 83312-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83312-1-RR (COPS8 antibody) at dilution of 1:250 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83312-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using COPS8 Recombinant antibody (83312-1-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83312-1-RR (same clone as 83312-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83312-1-RR (COPS8 antibody) at dilution of 1:250 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83312-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using COPS8 Recombinant antibody (83312-1-RR)

    IF Staining of SH-SY5Y using 83312-1-RR (same clone as 83312-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using COPS8 antibody (83312-1-RR, Clone: 240195B1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83312-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00336-2

    Cytometric bead array standard curve of MP00336-2

    Cytometric bead array standard curve of MP00336-2, COPS8 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83312-2-PBS. Detection antibody: 83312-1-PBS. Standard: Ag0143. Range: 0.625-40 ng/mL.

  • Affinity and Kinetic Characterization of 83312-1-RR

    Affinity and Kinetic Characterization of 83312-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83312-1-RR against Human COPS8 were performed. The affinity constant is 1.67 nM.

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COPS8 Recombinant antibody, PBS Only (Detector)

COPS8 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 83312-1-PBS
Clone No.240195B1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

COP9, 240195B1, JAB1-containing signalosome subunit 8, hCOP9, CSN8

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83312-1-PBS targets COPS8 as part of a matched antibody pair:

MP00336-2: 83312-2-PBS capture and 83312-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen COPS8 fusion protein Ag0143 相同性解析による交差性が予測される生物種
Full Name COP9 constitutive photomorphogenic homolog subunit 8 (Arabidopsis)
Calculated molecular weight 23 kDa
Observed molecular weight23 kDa
GenBank accession numberBC003090
Gene Symbol COPS8
Gene ID (NCBI) 10920
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99627
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

COPS8 is one of the eight subunits of COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. The structure and function of COP9 signalosome is similar to that of the 19S regulatory particle of 26S proteasome. COP9 signalosome has been shown to interact with SCF-type E3 ubiquitin ligases and act as a positive regulator of E3 ubiquitin ligases.