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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

COPS8 Recombinant antibody

COPS8 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240195B1

Cat no : 83312-1-RR

Synonyms

COP9, 240195B1, JAB1-containing signalosome subunit 8, hCOP9, CSN8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COPS8 Recombinant antibody (83312-1-RR)

    WB analysis using 83312-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83312-1-RR (COPS8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using COPS8 Recombinant antibody (83312-1-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83312-1-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83312-1-RR (COPS8 antibody) at dilution of 1:250 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using COPS8 Recombinant antibody (83312-1-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83312-1-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83312-1-RR (COPS8 antibody) at dilution of 1:250 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using COPS8 Recombinant antibody (83312-1-RR)

    IF Staining of SH-SY5Y using 83312-1-RR

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using COPS8 antibody (83312-1-RR, Clone: 240195B1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83312-1-RR

    Affinity and Kinetic Characterization of 83312-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83312-1-RR against Human COPS8 were performed. The affinity constant is 1.67 nM.

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Tested Applications

Positive WB detected inSH-SY5Y cells, HepG2 cells, T-47D cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:125-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83312-1-RR is COPS8 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen COPS8 fusion protein Ag0143 相同性解析による交差性が予測される生物種
Full Name COP9 constitutive photomorphogenic homolog subunit 8 (Arabidopsis)
Calculated molecular weight 23 kDa
Observed molecular weight23 kDa
GenBank accession numberBC003090
Gene symbol COPS8
Gene ID (NCBI) 10920
RRIDAB_3670979
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COPS8 is one of the eight subunits of COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. The structure and function of COP9 signalosome is similar to that of the 19S regulatory particle of 26S proteasome. COP9 signalosome has been shown to interact with SCF-type E3 ubiquitin ligases and act as a positive regulator of E3 ubiquitin ligases.

Protocols

Product Specific Protocols
WB protocol for COPS8 antibody 83312-1-RRDownload protocol
IHC protocol for COPS8 antibody 83312-1-RRDownload protocol
IF protocol for COPS8 antibody 83312-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols