Validation Data Gallery

Filter: AllWBIPIHCIF-PIF-FroFC (Intra)

WB analysis of rat brain using 17760-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 17760-1-AP (CORO1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 17760-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17760-1-AP (CORO1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of mouse spleen using 17760-1-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 17760-1-AP (CORO1A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 17760-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 17760-1-AP (CORO1A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 17760-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 17760-1-AP (CORO1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of rat spleen using 17760-1-AP
rat spleen tissue were subjected to SDS PAGE followed by western blot with 17760-1-AP (CORO1A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 17760-1-AP
IP result of anti-CORO1A (IP:17760-1-AP, 4ug; Detection:17760-1-AP 1:3000) with mouse brain tissue lysate 1840 ug.
IP experiment of mouse spleen using 17760-1-AP
IP result of anti-CORO1A (IP:17760-1-AP, 4ug; Detection:17760-1-AP 1:1000) with mouse spleen tissue lysate 4800ug.
IHC staining of mouse spleen using 17760-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 17760-1-AP (CORO1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse spleen using 17760-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 17760-1-AP (CORO1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat spleen using 17760-1-AP
Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 17760-1-AP (CORO1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat spleen using 17760-1-AP
Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 17760-1-AP (CORO1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse spleen using 17760-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CORO1A antibody (17760-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse colon using 17760-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using CORO1A antibody (17760-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse spleen using 17760-1-AP
Immunofluorescent analysis of frozen OCT-embedded mouse spleen tissue using CORO1A antibody (17760-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
FC experiment of Jurkat using 17760-1-AP
1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human CORO1A (17760-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CORO1A Polyclonal antibody

CORO1A Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, FC (Intra), IP, ELISA

Cat No. 17760-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IF-Fro, FC (Intra), IP, ELISA

Synonyms

Coronin 1A, CORO1, Clipin-A, CLIPINA, Clipin A

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (5)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse brain tissue, HL-60 cells, Jurkat cells, mouse spleen tissue, rat brain tissue, rat spleen tissue, mouse thymus tissue
Positive IP detected in	mouse brain tissue, mouse spleen tissue
Positive IHC detected in	rat spleen tissue, mouse spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse spleen tissue, mouse colon tissue
Positive IF-Fro detected in	mouse spleen tissue
Positive FC (Intra) detected in	Jurkat cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)-FRO	IF-FRO : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IF	See 1 publications below

Product Information

17760-1-AP targets CORO1A in WB, IHC, IF-P, IF-Fro, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CORO1A fusion protein Ag12139 相同性解析による交差性が予測される生物種
Full Name	coronin, actin binding protein, 1A
Calculated molecular weight	461 aa, 51 kDa
Observed molecular weight	57 kDa
GenBank accession number	BC110374
Gene Symbol	CORO1A
Gene ID (NCBI)	11151
RRID	AB_2082070
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P31146
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CORO1A also known as TACO, belongs to the WD repeat coronin family. CORO1A is recruited to phagosomes and actively retained by surviving mycobacteria and is usually released before phagosome fusion with or maturation into lysosomes. CORO1A is expressed in the brain, thymus, spleen, bone marrow, and lymph nodes. Mutations in CORO1A cause Immunodeficiency 8 with lymphoproliferation (IMD8)(PMID: 10338208).

Protocols

Product Specific Protocols
WB protocol for CORO1A antibody 17760-1-AP	Download protocol
IHC protocol for CORO1A antibody 17760-1-AP	Download protocol
IF protocol for CORO1A antibody 17760-1-AP	Download protocol
IP protocol for CORO1A antibody 17760-1-AP	Download protocol
FC protocol for CORO1A antibody 17760-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
		Br J Pharmacol PMID: 27128203 Authors - Xianrong Zhang View Article
human	WB	Int Immunopharmacol Carbamazepine-modified HLA-A*24:02-bound peptidome: Implication of CORO1A in skin rash. Authors - Fuli Min View Article
human	WB	Front Med (Lausanne) MCC Regulator of WNT Signaling Pathway (MCC) Is a Podocyte Essential Gene. Authors - Hui Song View Article
rat	WB	PLoS One Repetitive Transcranial Magnetic Stimulation Promotes Neural Stem Cell Proliferation via the Regulation of MiR-25 in a Rat Model of Focal Cerebral Ischemia. Authors - Feng Guo View Article
human	IF	Theranostics TRIM4 enhances small-molecule-induced neddylated-degradation of CORO1A for triple negative breast cancer therapy Authors - Wen-Jie Gu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of rat brain using 17760-1-AP

WB analysis using 17760-1-AP

WB analysis of mouse spleen using 17760-1-AP

WB analysis of Jurkat using 17760-1-AP

WB analysis of HL-60 using 17760-1-AP

WB analysis of rat spleen using 17760-1-AP

IP experiment of mouse brain using 17760-1-AP

IP experiment of mouse spleen using 17760-1-AP

IHC staining of mouse spleen using 17760-1-AP

IHC staining of mouse spleen using 17760-1-AP

IHC staining of rat spleen using 17760-1-AP

IHC staining of rat spleen using 17760-1-AP

IF Staining of mouse spleen using 17760-1-AP

IF Staining of mouse colon using 17760-1-AP

IF Staining of mouse spleen using 17760-1-AP

FC experiment of Jurkat using 17760-1-AP

CORO1A Polyclonal antibody

CORO1A Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

PMID: 27128203

Carbamazepine-modified HLA-A*24:02-bound peptidome: Implication of CORO1A in skin rash.

MCC Regulator of WNT Signaling Pathway (MCC) Is a Podocyte Essential Gene.

Repetitive Transcranial Magnetic Stimulation Promotes Neural Stem Cell Proliferation via the Regulation of MiR-25 in a Rat Model of Focal Cerebral Ischemia.

TRIM4 enhances small-molecule-induced neddylated-degradation of CORO1A for triple negative breast cancer therapy