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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated CORO1A Polyclonal antibody

CORO1A Polyclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-17760

Synonyms

Coronin 1A, CORO1, Clipin-A, CLIPINA, Clipin A

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human colon tissue using CoraLite® Plus 488-conjugated CORO1A Polyclonal an (CL488-17760)

    IF Staining of human colon using CL488-17760

    Immunofluorescent analysis of (4% PFA) fixed human colon tissue using CoraLite® Plus 488 CORO1A antibody (CL488-17760) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human colon tissue using CoraLite® Plus 488-conjugated CORO1A Polyclonal an (CL488-17760)

    IF Staining of human colon using CL488-17760

    Immunofluorescent analysis of (4% PFA) fixed human colon tissue using CoraLite® Plus 488 CORO1A antibody (CL488-17760) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using CoraLite® Plus 488-conjugated CORO1A Polyclonal an (CL488-17760)

    IF Staining of mouse spleen using CL488-17760

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CoraLite® Plus 488 CORO1A antibody (CL488-17760) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using CoraLite® Plus 488-conjugated CORO1A Polyclonal an (CL488-17760)

    IF Staining of mouse spleen using CL488-17760

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CoraLite® Plus 488 CORO1A antibody (CL488-17760) at dilution of 1:200.

  • Flow cytometry (FC) experiment of Jurkat cells using CoraLite® Plus 488-conjugated CORO1A Polyclonal an (CL488-17760)

    FC experiment of Jurkat using CL488-17760

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CORO1A (CL488-17760) (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF-P detected inhuman colon tissue, mouse spleen tissue
Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-17760 targets CORO1A in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CORO1A fusion protein Ag12139 相同性解析による交差性が予測される生物種
Full Name coronin, actin binding protein, 1A
Calculated molecular weight 461 aa, 51 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC110374
Gene symbol CORO1A
Gene ID (NCBI) 11151
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CORO1A antibody CL488-17760Download protocol
Standard Protocols
Click here to view our Standard Protocols