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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CORO1A Recombinant antibody (84602-5-RR)

    WB analysis using 84602-5-RR (same clone as 84602-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84602-5-RR (CORO1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84602-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CORO1A Recombinant antibody (84602-5-RR)

    IF Staining of Jurkat using 84602-5-RR (same clone as 84602-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CORO1A antibody (84602-5-RR, Clone: 242027H5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84602-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Jurkat cells using CORO1A Recombinant antibody (84602-5-RR)

    FC experiment of Jurkat using 84602-5-RR (same clone as 84602-5-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug CORO1A Recombinant antibody (84602-5-RR, Clone:242027H5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84602-5-PBS in a different storage buffer formulation.

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CORO1A Recombinant antibody, PBS Only

CORO1A Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84602-5-PBS
Clone No.242027H5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

CORO1, Clipin-A, CLIPINA, Clipin A, CLABP

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84602-5-PBS targets CORO1A in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CORO1A fusion protein Ag12139 相同性解析による交差性が予測される生物種
Full Name coronin, actin binding protein, 1A
Calculated molecular weight 461 aa, 51 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC110374
Gene Symbol CORO1A
Gene ID (NCBI) 11151
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP31146
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CORO1A also known as TACO, belongs to the WD repeat coronin family. CORO1A is recruited to phagosomes and actively retained by surviving mycobacteria and is usually released before phagosome fusion with or maturation into lysosomes. CORO1A is expressed in the brain, thymus, spleen, bone marrow, and lymph nodes. Mutations in CORO1A cause Immunodeficiency 8 with lymphoproliferation (IMD8)(PMID: 10338208).