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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CORO1A Recombinant antibody (84602-5-RR)

    WB analysis using 84602-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84602-5-RR (CORO1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CORO1A Recombinant antibody (84602-5-RR)

    IF Staining of Jurkat using 84602-5-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CORO1A antibody (84602-5-RR, Clone: 242027H5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of Jurkat cells using CORO1A Recombinant antibody (84602-5-RR)

    FC experiment of Jurkat using 84602-5-RR

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug CORO1A Recombinant antibody (84602-5-RR, Clone:242027H5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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CORO1A Recombinant antibody

CORO1A Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84602-5-RR
Clone No.242027H5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

CORO1, Clipin-A, CLIPINA, Clipin A, CLABP

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, RAW 264.7 cells, mouse brain tissue, rat brain tissue
Positive IF/ICC detected inJurkat cells
Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84602-5-RR targets CORO1A in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CORO1A fusion protein Ag12139 相同性解析による交差性が予測される生物種
Full Name coronin, actin binding protein, 1A
Calculated molecular weight 461 aa, 51 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC110374
Gene Symbol CORO1A
Gene ID (NCBI) 11151
RRIDAB_3672091
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP31146
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CORO1A also known as TACO, belongs to the WD repeat coronin family. CORO1A is recruited to phagosomes and actively retained by surviving mycobacteria and is usually released before phagosome fusion with or maturation into lysosomes. CORO1A is expressed in the brain, thymus, spleen, bone marrow, and lymph nodes. Mutations in CORO1A cause Immunodeficiency 8 with lymphoproliferation (IMD8)(PMID: 10338208).

Protocols

Product Specific Protocols
WB protocol for CORO1A antibody 84602-5-RRDownload protocol
IF protocol for CORO1A antibody 84602-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols