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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CORO1C Monoclonal antibody (68846-1-Ig)

    WB analysis using 68846-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68846-1-Ig (CORO1C antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CORO1C Monoclonal antibody (68846-1-Ig)

    WB analysis using 68846-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68846-1-Ig (CORO1C antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using CORO1C Monoclonal antibody (68846-1-Ig)

    IF Staining of SH-SY5Y using 68846-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CORO1C antibody (68846-1-Ig, Clone: 1B10G4 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

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CORO1C Monoclonal antibody

CORO1C Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68846-1-Ig
Clone No.1B10G4

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

CRN2, Coronin-3, Coronin-1C, Coronin 3, Coronin 1C

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhTERT-RPE1 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, MDA-MB-231 cells, A549 cells, A431 cells, HepG2 cells
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68846-1-Ig targets CORO1C in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CORO1C fusion protein Ag6546 相同性解析による交差性が予測される生物種
Full Name coronin, actin binding protein, 1C
Calculated molecular weight 53 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC002342
Gene Symbol CORO1C
Gene ID (NCBI) 23603
RRIDAB_3670439
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9ULV4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CORO1C also known as HCRNN4, belongs to the WD repeat coronin family. CORO1C contains 5 N-terminal trp-asp (WD) repeats and a C-terminal coiled-coil motif. It was reported to regulate actin‐dependent processes by assembling F-actin. CORO1C promoted the invasion of breast cancer and glioma cells by specifically promoting the formation of invadopodia(PMID: 30974047).

Protocols

Product Specific Protocols
WB protocol for CORO1C antibody 68846-1-IgDownload protocol
IF protocol for CORO1C antibody 68846-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols