SARS-CoV-2 S protein (RBD, 319-541 aa) Monoclonal antibody
SARS-CoV-2 S protein (RBD, 319-541 aa) Monoclonal Antibody for WB, ELISA, Neutralization
Host / Isotype
Mouse / IgG1
Reactivity
Virus and More (2)
Applications
WB, Neutralization, ELISA
Conjugate
Unconjugated
CloneNo.
1H3E9
Cat no : 67758-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Ag30688 |
| Positive Neutralization detected in | Recombinant protein |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| NEUTRALIZATION | NEUTRALIZATION : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 8 publications below |
Product Information
67758-1-Ig targets SARS-CoV-2 S protein (RBD, 319-541 aa) in WB, Neutralization, ELISA applications and shows reactivity with Virus samples.
| Tested Reactivity | Virus |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | SARS-CoV-2 S protein (RBD, 319-541 aa) fusion protein Ag30688 相同性解析による交差性が予測される生物種 |
| Full Name | SARS-CoV-2 Spike Protein |
| Calculated molecular weight | 141 kDa |
| GenBank accession number | NC_045512 |
| Gene symbol | SARS-COV-2 |
| Gene ID (NCBI) | 43740568 |
| RRID | AB_2918526 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Coronaviruses (CoVs) infect human and animals and cause varieties of diseases, including respiratory, enteric, renal, and neurological diseases. CoV uses its spike protein to recognize ACE2 as its receptors and mediate membrane fusion and virus entry into host cells(PMID: 32221306). Each monomer of trimeric S protein is about 180 kDa, and contains two subunits, S1 and S2,S1 recognizes and binds to host receptors, and subsequent conformational changes in S2 facilitate fusion between the viral envelope and the host cell membrane(PMID: 19198616). Although the amino acid sequences of the S-glycoprotein were found to be different between the various HCoV, the structures showed high similarity, but the best 3D structural overlap shared by SARS-CoV and SARS-CoV-2, consistent with the shared ACE2 predicted receptor (PMID: 32522207). The spike protein of CoVs can be a target for vaccine and therapeutic development (PMID: 19198616).
Lyophilized format of this product is available.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SARS-CoV-2 S protein (RBD, 319-541 aa) antibody 67758-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Acta Pharm Sin B A novel heterologous receptor-binding domain dodecamer universal mRNA vaccine against SARS-CoV-2 variants | ||
J Med Virol A novel three-plasmid packaging system for chimeric SFV/SIN VRPs derived from Semliki Forest virus and Sindbis virus as a candidate gene delivery vector | ||
bioRxiv Novel sensor-integrated proteome on chip (SPOC) platform with thousands of folded proteins on a 1.5 sq-cm biosensor chip to enable high-throughput real-time label-free screening for kinetic analysis | ||
Virol J LINC01002 functions as a ceRNA to regulate FRMD8 by sponging miR-4324 for the development of COVID-19 | ||
Int J Mol Sci SARS-CoV-2 Rapidly Infects Peripheral Sensory and Autonomic Neurons, Contributing to Central Nervous System Neuroinvasion before Viremia |
