Virus SARS-CoV-2 S protein (RBD, 319-541 aa) Monoclonal antibody, PBS Only

Recombinant spike protein (RBD domain) were subjected to SDS PAGE followed by western blot with 67758-1-lg (SARS-CoV-2 Spike Glycoprotein (319-541 aa) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67758-1-PBS in a different storage buffer formulation.

Surrogate virus neutralization test of SARS-CoV-2 Spike Glycoprotein (319-541 aa) monoclonal antibody 67758-1-Ig using commercial kit. Briefly, RBD protein has been pre-coated on microplate, HRP labeled ACE2 protein and serial dose of 67758-1-Ig are added to the plate simultaneously and incubated for 1 hour at 37°C. The plate was then washed and signal was developed by adding chromogenic substrate followed by stop buffer. Signal strength was monitored at absorbance of 450nm. The IC50 is around 20 μg/mL. Please note that different IC50 will be obtained if different assays are used. Lyophilized format of this product is available. This data was developed using the same antibody clone with 67758-1-PBS in a different storage buffer formulation.