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Validation Data Gallery

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  • Western Blot (WB) analysis of Recombinant protein using virus SARS-CoV-2 S protein (RBD, 319-541 aa) Monoc (67758-1-Ig)

    WB analysis of Recombinant protein using 67758-1-Ig (same clone as 67758-1-PBS)

    Recombinant spike protein (RBD domain) were subjected to SDS PAGE followed by western blot with 67758-1-lg (SARS-CoV-2 Spike Glycoprotein (319-541 aa) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67758-1-PBS in a different storage buffer formulation.

  • Neutralization experiment of SARS-CoV-2 S protein (RBD, 319-541 aa) using virus SARS-CoV-2 S protein (RBD, 319-541 aa) Monoc (67758-1-Ig)

    Neutralization experiment of SARS-CoV-2 S protein (RBD, 319-541 aa) using 67758-1-Ig (same clone as 67758-1-PBS)

    Surrogate virus neutralization test of SARS-CoV-2 Spike Glycoprotein (319-541 aa) monoclonal antibody 67758-1-Ig using commercial kit. Briefly, RBD protein has been pre-coated on microplate, HRP labeled ACE2 protein and serial dose of 67758-1-Ig are added to the plate simultaneously and incubated for 1 hour at 37°C. The plate was then washed and signal was developed by adding chromogenic substrate followed by stop buffer. Signal strength was monitored at absorbance of 450nm. The IC50 is around 20 μg/mL. Please note that different IC50 will be obtained if different assays are used. Lyophilized format of this product is available. This data was developed using the same antibody clone with 67758-1-PBS in a different storage buffer formulation.

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Virus SARS-CoV-2 S protein (RBD, 319-541 aa) Monoclonal antibody, PBS Only

SARS-CoV-2 S protein (RBD, 319-541 aa) Monoclonal Antibody for WB, Neutralization, Indirect ELISA
Cat No. 67758-1-PBS
Clone No.1H3E9

Host / Isotype

Mouse / IgG1

Reactivity

Virus

Applications

WB, Neutralization, Indirect ELISA

Synonyms

COVID-19 RBD, RBD, SARS-CoV-2 Spike Glycoprotein (319-541 aa), spike protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67758-1-PBS targets SARS-CoV-2 S protein (RBD, 319-541 aa) in WB, Neutralization, Indirect ELISA applications and shows reactivity with Virus samples.

Tested Reactivity Virus
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SARS-CoV-2 S protein (RBD, 319-541 aa) fusion protein Ag30688 相同性解析による交差性が予測される生物種
Full Name SARS-CoV-2 Spike Protein
Calculated molecular weight 141 kDa
GenBank accession numberNC_045512
Gene Symbol SARS-COV-2
Gene ID (NCBI) 43740568
RRIDAB_2918526
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Coronaviruses (CoVs) infect human and animals and cause varieties of diseases, including respiratory, enteric, renal, and neurological diseases. CoV uses its spike protein to recognize ACE2 as its receptors and mediate membrane fusion and virus entry into host cells(PMID: 32221306). Each monomer of trimeric S protein is about 180 kDa, and contains two subunits, S1 and S2,S1 recognizes and binds to host receptors, and subsequent conformational changes in S2 facilitate fusion between the viral envelope and the host cell membrane(PMID: 19198616). Although the amino acid sequences of the S-glycoprotein were found to be different between the various HCoV, the structures showed high similarity, but the best 3D structural overlap shared by SARS-CoV and SARS-CoV-2, consistent with the shared ACE2 predicted receptor (PMID: 32522207). The spike protein of CoVs can be a target for vaccine and therapeutic development (PMID: 19198616).

Lyophilized format of this product is available.