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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COX-1/Cyclooxygenase-1 Recombinant antibody (84151-4-RR)

    WB analysis using 84151-4-RR (same clone as 84151-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84151-4-RR (COX-1/Cyclooxygenase-1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84151-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of THP-1 cells using COX-1/Cyclooxygenase-1 Recombinant antibody (84151-4-RR)

    WB analysis of THP-1 using 84151-4-RR (same clone as 84151-4-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 84151-4-RR (COX-1/Cyclooxygenase-1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84151-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84151-4-RR

    Affinity and Kinetic Characterization of 84151-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84151-4-RR against Human COX-1/Cyclooxygenase-1 were performed. The affinity constant is below 1 pM.

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COX-1/Cyclooxygenase-1 Recombinant antibody, PBS Only

COX-1/Cyclooxygenase-1 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 84151-4-PBS
Clone No.241373C10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

PGH1, PTGS1, PGHS-1, EC:1.14.99.1, Cyclooxygenase-1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84151-4-PBS targets COX-1/Cyclooxygenase-1 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen COX-1/Cyclooxygenase-1 fusion protein Ag4055 相同性解析による交差性が予測される生物種
Full Name prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase)
Calculated molecular weight 599 aa, 69 kDa
Observed molecular weight 60-72 kDa
GenBank accession numberBC029840
Gene Symbol PTGS1
Gene ID (NCBI) 5742
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP23219
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PTGS1(Prostaglandin G/H synthase 1) belongs to the prostaglandin G/H synthase family and catalyzes the conversion of arachidonic acid to prostaglandin H2, which is subsequently metabolized to various biologically active prostaglandins. It is also named as COX1(Cyclooxygenase-1). There is no PTGS1 expression in fetal samples (prostate, seminal vesicles, or ejaculatory ducts), and only minimal expression in adult tissues (PMID: 10999846). PTGS1 has some isoforms with MW of 56-72 kD and PTGS1 can form homodimer.