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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

COX-1/Cyclooxygenase-1 Recombinant antibody

COX-1/Cyclooxygenase-1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241373C10

Cat no : 84151-4-RR

Synonyms

PGH1, PTGS1, PGHS-1, EC:1.14.99.1, Cyclooxygenase-1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COX-1/Cyclooxygenase-1 Recombinant antibody (84151-4-RR)

    WB analysis using 84151-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84151-4-RR (COX-1/Cyclooxygenase-1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of THP-1 cells using COX-1/Cyclooxygenase-1 Recombinant antibody (84151-4-RR)

    WB analysis of THP-1 using 84151-4-RR

    THP-1 cells were subjected to SDS PAGE followed by western blot with 84151-4-RR (COX-1/Cyclooxygenase-1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84151-4-RR

    Affinity and Kinetic Characterization of 84151-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84151-4-RR against Human COX-1/Cyclooxygenase-1 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inC2C12 cells, THP-1 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84151-4-RR is COX-1/Cyclooxygenase-1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen COX-1/Cyclooxygenase-1 fusion protein Ag4055 相同性解析による交差性が予測される生物種
Full Name prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase)
Calculated molecular weight 599 aa, 69 kDa
Observed molecular weight 60-72 kDa
GenBank accession numberBC029840
Gene symbol PTGS1
Gene ID (NCBI) 5742
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PTGS1(Prostaglandin G/H synthase 1) belongs to the prostaglandin G/H synthase family and catalyzes the conversion of arachidonic acid to prostaglandin H2, which is subsequently metabolized to various biologically active prostaglandins. It is also named as COX1(Cyclooxygenase-1). There is no PTGS1 expression in fetal samples (prostate, seminal vesicles, or ejaculatory ducts), and only minimal expression in adult tissues (PMID: 10999846). PTGS1 has some isoforms with MW of 56-72 kD and PTGS1 can form homodimer.

Protocols

Product Specific Protocols
WB protocol for COX-1/Cyclooxygenase-1 antibody 84151-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols