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COX2/ Cyclooxygenase 2/ PTGS2 Monoclonal antibody

COX2/ Cyclooxygenase 2/ PTGS2 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

CloneNo.

3G2B9

Cat no : 66351-1-Ig

Synonyms

COX2, PTGS2, 3G2B9, EC:1.14.99.1, Cyclooxygenase-2


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Tested Applications

Positive WB detected inRAW 264.7 cells, LPS treated HeLa cells, LPS treated RAW 264.7 cells
Positive IHC detected inhuman cervical cancer tissue, human breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse lung tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66351-1-Ig targets COX2/ Cyclooxygenase 2/ PTGS2 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen COX2/ Cyclooxygenase 2/ PTGS2 fusion protein Ag24721 相同性解析による交差性が予測される生物種
Full Name prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
Calculated molecular weight 604 aa, 68 kDa
Observed molecular weight 66-70 kDa
GenBank accession numberBC013734
Gene symbol COX2/PTGS2
Gene ID (NCBI) 5743
RRIDAB_2881731
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COX2 is an enzyme involved in the synthesis of prostaglandins from arachidonic acid.


1. What is the molecular weight of COX2?

The fully N-glycosylated PTGS2 is 72-74 kDa and the aglycosylated is 66 kDa (PMID:19656660). It also 

expresses a band of 39 kDa after unspecific cleavage (PMID:17509125). The 50 kDa band of fragmented 

PTGS2 has also previously been detected in AD brains (PMID:14724276).

2. Is COX2 post-translationally modified?

COX2 is a subject of post-translational modifications, including phosphorylation, glycosylation, and 

s-nitrosylation (PMID: 28939645).

3. What is the difference between COX1, COX2, and COX3?

There are three isoenzymes that have cyclooxygenase activity: COX1, COX2, and COX3. While COX1 

is a ubiquitously expressed constitutive enzyme, expression of COX2 is generally low but can be rapidly 

induced by various stimuli (as part of infection and inflammatory response) and is controlled by the 

transcription factor NFκB. COX3 is an alternative splice variant of COX1 enzyme and is considered 

non-functional in humans. 

4. I cannot detect COX2 in my sample during western blotting.

Unlike COX1, COX2 expression is inducible by a variety of stimuli including certain growth factors, cytokines, 

and proinflammatory stimuli. COX2 basal expression levels may be very low in unstimulated cells. Additionally, 

the COX2 half-life time is short and after stimulation, COX2 protein can be quickly degraded to basal levels 

(PMID: 10966456), which should be taken into account during experimental design.

5. What is the role of COX2 in cancer?

COX2 is often upregulated in various cancer types and increased expression of COX2 is associated with 

greater angiogenesis of solid tumors, increased invasion, and metastasis, as well as decreased host immunity.

6. What is subcellular localization of COX2?

Both COX1 and COX2 are present in the endoplasmic reticulum (ER) and nuclear envelope (PMID: 9545330), 

but COX2 has been reported to be more enriched in the nuclear envelope compared to COX1 (PMID: 7738031).


Protocols

Product Specific Protocols
WB protocol for COX2/ Cyclooxygenase 2/ PTGS2 antibody 66351-1-IgDownload protocol
IHC protocol for COX2/ Cyclooxygenase 2/ PTGS2 antibody 66351-1-IgDownload protocol
IF protocol for COX2/ Cyclooxygenase 2/ PTGS2 antibody 66351-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Metab

Cooperation between COA6 and SCO2 in COX2 maturation during cytochrome c oxidase assembly links two mitochondrial cardiomyopathies.

Authors - David Pacheu-Grau
IHC

ACS Nano

Engineered Extracellular Vesicle-Delivered CRISPR/Cas9 for Radiotherapy Sensitization of Glioblastoma

Authors - Xiao Liu
mouseWB

Adv Sci (Weinh)

Tryptophan As a New Member of RNA-Induced Silencing Complexes Prevents Colon Cancer Liver Metastasis

Authors - Fangyi Xu
humanWB

Redox Biol

The deubiquitinase USP11 ameliorates intervertebral disc degeneration by regulating oxidative stress-induced ferroptosis via deubiquitinating and stabilizing Sirt3

Authors - Jian Zhu
mouseWB

J Nanobiotechnology

Injectable photocrosslinking spherical hydrogel-encapsulated targeting peptide-modified engineered exosomes for osteoarthritis therapy

Authors - Junlai Wan
mouseIHC

J Nanobiotechnology

Low-dose celecoxib-loaded PCL fibers reverse intervertebral disc degeneration by up-regulating CHSY3 expression

Authors - Yunhao Wang