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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

COX4NB Monoclonal antibody, PBS Only

COX4NB Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B8D3

Cat no : 66547-1-PBS

Synonyms

C16orf2, C16orf4, COX4 neighbor, COX4AL, COX4NB, FAM158B, Neighbor of COX4, NOC4, Protein FAM158B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COX4NB Monoclonal antibody (66547-1-Ig)

    WB analysis using 66547-1-Ig (same clone as 66547-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66547-1-Ig (COX4NB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66547-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using COX4NB Monoclonal antibody (66547-1-Ig)

    IHC staining of human liver using 66547-1-Ig (same clone as 66547-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66547-1-Ig (COX4NB antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66547-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using COX4NB Monoclonal antibody (66547-1-Ig)

    IHC staining of human kidney using 66547-1-Ig (same clone as 66547-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66547-1-Ig (COX4NB antibody) at dilution of 1:400 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66547-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using COX4NB Monoclonal antibody (66547-1-Ig)

    IF Staining of HeLa using 66547-1-Ig (same clone as 66547-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using COX4NB antibody (66547-1-Ig, Clone: 2B8D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66547-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66547-1-PBS targets COX4NB in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen COX4NB fusion protein Ag13776 相同性解析による交差性が予測される生物種
Full Name COX4 neighbor
Calculated molecular weight 210 aa, 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC001472
Gene symbol COX4NB
Gene ID (NCBI) 10328
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.