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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COX4NB Monoclonal antibody (66547-1-Ig)

    WB analysis using 66547-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66547-1-Ig (COX4NB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using COX4NB Monoclonal antibody (66547-1-Ig)

    IHC staining of human liver using 66547-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66547-1-Ig (COX4NB antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using COX4NB Monoclonal antibody (66547-1-Ig)

    IHC staining of human kidney using 66547-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66547-1-Ig (COX4NB antibody) at dilution of 1:400 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using COX4NB Monoclonal antibody (66547-1-Ig)

    IF Staining of HeLa using 66547-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using COX4NB antibody (66547-1-Ig, Clone: 2B8D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

COX4NB Monoclonal antibody

COX4NB Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66547-1-Ig
Clone No.2B8D3
Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

C16orf2, C16orf4, COX4 neighbor, COX4AL, COX4NB, FAM158B, Neighbor of COX4, NOC4, Protein FAM158B

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman liver tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66547-1-Ig targets COX4NB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen COX4NB fusion protein Ag13776 相同性解析による交差性が予測される生物種
Full Name COX4 neighbor
Calculated molecular weight 210 aa, 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC001472
Gene Symbol COX4NB
Gene ID (NCBI) 10328
RRIDAB_2881909
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO43402
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for COX4NB antibody 66547-1-IgDownload protocol
IHC protocol for COX4NB antibody 66547-1-IgDownload protocol
IF protocol for COX4NB antibody 66547-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

iScience

The endoplasmic reticulum membrane complex promotes proteostasis of GABAA receptors

Authors - Angela L. Whittsette
View Article