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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated COX4NB Monoclonal antibody

COX4NB Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B8D3

Cat no : CL488-66547

Synonyms

EMC8, ER membrane protein complex subunit 8, COX4AL, COX4 neighbor, C16orf4

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated COX4NB Monoclonal an (CL488-66547)

    IF Staining of HeLa using CL488-66547

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 COX4NB antibody (CL488-66547, Clone: 2B8D3 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated COX4NB Monoclonal an (CL488-66547)

    FC experiment of HepG2 using CL488-66547

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human COX4NB (CL488-66547, Clone:2B8D3) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66547 targets COX4NB in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen COX4NB fusion protein Ag13776 相同性解析による交差性が予測される生物種
Full Name COX4 neighbor
Calculated molecular weight 210 aa, 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC001472
Gene symbol COX4NB
Gene ID (NCBI) 10328
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 COX4NB antibody CL488-66547Download protocol
Standard Protocols
Click here to view our Standard Protocols