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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated COX5B Polyclonal ant (CL488-11418)

    IF Staining of HeLa using CL488-11418

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 COX5B antibody (CL488-11418) at dilution of 1:200, CL594-Phalloidin (red).

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  • KD/KO Validated

CoraLite® Plus 488-conjugated COX5B Polyclonal antibody

COX5B Polyclonal Antibody for IF/ICC
Cat No. CL488-11418

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

COX5B, COXVB

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-11418 targets COX5B in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COX5B fusion protein Ag1986 相同性解析による交差性が予測される生物種
Full Name cytochrome c oxidase subunit Vb
Calculated molecular weight 129 aa, 14 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC006229
Gene Symbol COX5B
Gene ID (NCBI) 1329
RRIDAB_3672495
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP10606
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COX5B belongs to the cytochrome c oxidase subunit 5B family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX5B and other 3 genes CKB, HBB, MBP are involved in respiration.(PMID:21295140)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 COX5B antibody CL488-11418Download protocol
Standard Protocols
Click here to view our Standard Protocols