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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated COXIV Recombinant antibody

COXIV Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3K20

Cat no : CL488-82916

Synonyms

COX4I1, Cytochrome c oxidase subunit 4 isoform 1, mitochondrial, COX4, COX IV-1, 3K20

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated COXIV Recombinant an (CL488-82916)

    IF Staining of HepG2 using CL488-82916

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 COXIV antibody (CL488-82916, Clone: 3K20 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated COXIV Recombinant an (CL488-82916)

    FC experiment of HepG2 using CL488-82916

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated COXIV Recombinant antibody (CL488-82916, Clone:3K20)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-82916 targets COXIV in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen COXIV fusion protein Ag1640 相同性解析による交差性が予測される生物種
Full Name cytochrome c oxidase subunit IV isoform 1
Calculated molecular weight19.6 kDa
Observed molecular weight17-18 kDa
GenBank accession numberBC021236
Gene symbol COX IV
Gene ID (NCBI) 1327
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COX4I1, also named as COX4 and COXIV-1, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX4I1 is a marker for mitochondria. It has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 COXIV antibody CL488-82916Download protocol
FC protocol for CL Plus 488 COXIV antibody CL488-82916Download protocol
Standard Protocols
Click here to view our Standard Protocols