CPLX2 Monoclonal antibody
CPLX2 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat, pig
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
1B2A11
Cat no : 66849-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
66849-1-Ig targets CPLX2 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, pig samples.
Tested Reactivity | Human, Mouse, Rat, pig |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | CPLX2 fusion protein Ag27949 相同性解析による交差性が予測される生物種 |
Full Name | complexin 2 |
Calculated molecular weight | 134 aa, 15 kDa |
Observed molecular weight | 18-20 kDa |
GenBank accession number | BC093706 |
Gene symbol | CPLX2 |
Gene ID (NCBI) | 10814 |
RRID | AB_2882189 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Complexins are soluble proteins that regulate the activity of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes necessary for vesicle fusion. Neuronal specific complexin 1 (CPLX1) has inhibitory and stimulatory effects on exocytosis by clamping trans-SNARE complexes in a prefusion state and promoting conformational changes to facilitate membrane fusion following cell stimulation. Complexin2 (CPLX2) is a pre-synaptic protein believed to regulate neurotransmitter release from pre-synaptic terminals, it is downregulated in schizophrenic patients suffering from depression, animal models of depression and neurological disorders such as Huntington's disease in which depression is a major symptom.
Protocols
Product Specific Protocols | |
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WB protocol for CPLX2 antibody 66849-1-Ig | Download protocol |
IHC protocol for CPLX2 antibody 66849-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |