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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CPLX2 Monoclonal antibody, PBS Only

CPLX2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B2A11

Cat no : 66849-1-PBS

Synonyms

921 L, complexin 2, Complexin II, CPLX2, CPX 2, CPX II, CPX2, Hfb1, Synaphin 1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using CPLX2 Monoclonal antibody (66849-1-Ig)

    WB analysis of mouse brain using 66849-1-Ig (same clone as 66849-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66849-1-Ig (CPLX2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66849-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using CPLX2 Monoclonal antibody (66849-1-Ig)

    WB analysis of rat brain using 66849-1-Ig (same clone as 66849-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66849-1-Ig (CPLX2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66849-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat cerebellum tissue using CPLX2 Monoclonal antibody (66849-1-Ig)

    WB analysis of rat cerebellum using 66849-1-Ig (same clone as 66849-1-PBS)

    rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 66849-1-Ig (CPLX2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66849-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse cerebellum tissue using CPLX2 Monoclonal antibody (66849-1-Ig)

    WB analysis of mouse cerebellum using 66849-1-Ig (same clone as 66849-1-PBS)

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 66849-1-Ig (CPLX2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66849-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CPLX2 Monoclonal antibody (66849-1-Ig)

    IHC staining of mouse brain using 66849-1-Ig (same clone as 66849-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66849-1-Ig (CPLX2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66849-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CPLX2 Monoclonal antibody (66849-1-Ig)

    IHC staining of mouse brain using 66849-1-Ig (same clone as 66849-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66849-1-Ig (CPLX2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66849-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66849-1-PBS targets CPLX2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, pig samples.

Tested Reactivity Human, Mouse, Rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CPLX2 fusion protein Ag27949 相同性解析による交差性が予測される生物種
Full Name complexin 2
Calculated molecular weight 134 aa, 15 kDa
Observed molecular weight 18-20 kDa
GenBank accession numberBC093706
Gene symbol CPLX2
Gene ID (NCBI) 10814
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Complexins are soluble proteins that regulate the activity of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes necessary for vesicle fusion. Neuronal specific complexin 1 (CPLX1) has inhibitory and stimulatory effects on exocytosis by clamping trans-SNARE complexes in a prefusion state and promoting conformational changes to facilitate membrane fusion following cell stimulation. Complexin2 (CPLX2) is a pre-synaptic protein believed to regulate neurotransmitter release from pre-synaptic terminals, it is downregulated in schizophrenic patients suffering from depression, animal models of depression and neurological disorders such as Huntington's disease in which depression is a major symptom.