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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CPLX2 Polyclonal antibody

CPLX2 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-18149

Synonyms

CPX II, CPX 2, CPLX 2, Complexin-2, 921L

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CoraLite® Plus 488-conjugated CPLX2 Polyclonal ant (CL488-18149)

    IF Staining of A549 using CL488-18149

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite® Plus 488 CPLX2 antibody (CL488-18149) at dilution of 1:200.

  • Flow cytometry (FC) experiment of A549 cells using CoraLite® Plus 488-conjugated CPLX2 Polyclonal ant (CL488-18149)

    FC experiment of A549 using CL488-18149

    1x10^6 A549 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated CPLX2 Polyclonal antibody (CL488-18149)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-18149 targets CPLX2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CPLX2 fusion protein Ag12838 相同性解析による交差性が予測される生物種
Full Name complexin 2
Calculated molecular weight 134 aa, 15 kDa
Observed molecular weight 18-20 kDa
GenBank accession numberBC093706
Gene symbol CPLX2
Gene ID (NCBI) 10814
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Complexins are soluble proteins that regulate the activity of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes necessary for vesicle fusion. Neuronal specific complexin 1 (CPLX1) has inhibitory and stimulatory effects on exocytosis by clamping trans-SNARE complexes in a prefusion state and promoting conformational changes to facilitate membrane fusion following cell stimulation. Complexin2 (CPLX2) is a pre-synaptic protein believed to regulate neurotransmitter release from pre-synaptic terminals, it is downregulated in schizophrenic patients suffering from depression, animal models of depression and neurological disorders such as Huntington's disease in which depression is a major symptom.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CPLX2 antibody CL488-18149Download protocol
FC protocol for CL Plus 488 CPLX2 antibody CL488-18149Download protocol
Standard Protocols
Click here to view our Standard Protocols