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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CPS1 Recombinant antibody (84277-1-RR)

    WB analysis of HeLa using 84277-1-RR (same clone as 84277-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 84277-1-RR (CPS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84277-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CPS1 Recombinant antibody (84277-1-RR)

    WB analysis using 84277-1-RR (same clone as 84277-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84277-1-RR (CPS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84277-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CPS1 Recombinant antibody (84277-1-RR)

    IHC staining of mouse liver using 84277-1-RR (same clone as 84277-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84277-1-RR (CPS1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84277-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CPS1 Recombinant antibody (84277-1-RR)

    IHC staining of mouse liver using 84277-1-RR (same clone as 84277-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84277-1-RR (CPS1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84277-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CPS1 Recombinant antibody (84277-1-RR)

    IF Staining of HepG2 using 84277-1-RR (same clone as 84277-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CPS1 antibody (84277-1-RR, Clone: 241198F1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84277-1-PBS in a different storage buffer formulation.

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CPS1 Recombinant antibody, PBS Only

CPS1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84277-1-PBS
Clone No.241198F1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

EC:6.3.4.16, CPSase I, Carbamoyl-phosphate synthetase I, Carbamoyl-phosphate synthase [ammonia], mitochondrial, 241198F1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84277-1-PBS targets CPS1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name carbamoyl-phosphate synthetase 1, mitochondrial
Calculated molecular weight 165 kDa
Observed molecular weight140-165 kDa
GenBank accession numberBC140943
Gene Symbol CPS1
Gene ID (NCBI) 1373
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP31327
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Carbamoyl phosphate synthetase 1 (CPS1) is the first rate-limiting enzyme in the urea cycle, converting ammonium into carbamoyl phosphate, and plays an intricate role in arginine metabolism and pyrimidine metabolism (PMID: 28376202). CPS1 expression is liver specific and suppressed in HCC cells and tumor tissues at the transcriptional level(PMID: 21281797). CPS1 gene comprises 38 exons that encode a polypeptide of 1500 amino acids and has a molecular weight of 165 kDa.