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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CPS1 Recombinant antibody

CPS1 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241198F1

Cat no : 84277-1-RR

Synonyms

EC:6.3.4.16, CPSase I, Carbamoyl-phosphate synthetase I, Carbamoyl-phosphate synthase [ammonia], mitochondrial, 241198F1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CPS1 Recombinant antibody (84277-1-RR)

    WB analysis of HeLa using 84277-1-RR

    HeLa cells were subjected to SDS PAGE followed by western blot with 84277-1-RR (CPS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CPS1 Recombinant antibody (84277-1-RR)

    WB analysis using 84277-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84277-1-RR (CPS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CPS1 Recombinant antibody (84277-1-RR)

    IF Staining of HepG2 using 84277-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CPS1 antibody (84277-1-RR, Clone: 241198F1 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inHeLa cells, mouse liver tissue, rat liver tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84277-1-RR targets CPS1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name carbamoyl-phosphate synthetase 1, mitochondrial
Calculated molecular weight 165 kDa
Observed molecular weight140-165 kDa
GenBank accession numberBC140943
Gene symbol CPS1
Gene ID (NCBI) 1373
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Carbamoyl phosphate synthetase 1 (CPS1) is the first rate-limiting enzyme in the urea cycle, converting ammonium into carbamoyl phosphate, and plays an intricate role in arginine metabolism and pyrimidine metabolism (PMID: 28376202). CPS1 expression is liver specific and suppressed in HCC cells and tumor tissues at the transcriptional level(PMID: 21281797). CPS1 gene comprises 38 exons that encode a polypeptide of 1500 amino acids and has a molecular weight of 165 kDa.

Protocols

Product Specific Protocols
WB protocol for CPS1 antibody 84277-1-RRDownload protocol
IF protocol for CPS1 antibody 84277-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols