Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84277-1-RR targets CPS1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | carbamoyl-phosphate synthetase 1, mitochondrial |
| Calculated molecular weight | 165 kDa |
| Observed molecular weight | 140-165 kDa |
| GenBank accession number | BC140943 |
| Gene Symbol | CPS1 |
| Gene ID (NCBI) | 1373 |
| RRID | AB_3671823 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P31327 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Carbamoyl phosphate synthetase 1 (CPS1) is the first rate-limiting enzyme in the urea cycle, converting ammonium into carbamoyl phosphate, and plays an intricate role in arginine metabolism and pyrimidine metabolism (PMID: 28376202). CPS1 expression is liver specific and suppressed in HCC cells and tumor tissues at the transcriptional level(PMID: 21281797). CPS1 gene comprises 38 exons that encode a polypeptide of 1500 amino acids and has a molecular weight of 165 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CPS1 antibody 84277-1-RR | Download protocol |
| IHC protocol for CPS1 antibody 84277-1-RR | Download protocol |
| WB protocol for CPS1 antibody 84277-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |