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  • Featured Product
  • KD/KO Validated

CPT1A Polyclonal antibody

CPT1A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 15184-1-AP

Synonyms

CPT1L, CPT-1A, CPT1, CPT I, Carnitine O-palmitoyltransferase I, liver isoform

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of A549 cells using CPT1A Polyclonal antibody (15184-1-AP)

    WB analysis of A549 cells using 15184-1-AP

    WB results of CPT1A antibody (1:1000) with shCPT1A-A549 and shCPT1A-HepG2 cells. shControl-A549 and shControl-HepG2 as positive control.

  • Western Blot (WB) analysis of various lysates using CPT1A Polyclonal antibody (15184-1-AP)

    WB analysis using 15184-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15184-1-AP (CPT1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CPT1A Polyclonal antibody (15184-1-AP)

    WB analysis using 15184-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15184-1-AP (CPT1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using CPT1A Polyclonal antibody (15184-1-AP)

    IP experiment of HepG2 using 15184-1-AP

    IP result of anti-CPT1A (IP:15184-1-AP, 4ug; Detection:15184-1-AP 1:1000) with HepG2 cells lysate 3000 ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CPT1A Polyclonal antibody (15184-1-AP)

    IHC staining of human liver cancer using 15184-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15184-1-AP (CPT1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CPT1A Polyclonal antibody (15184-1-AP)

    IHC staining of mouse brain using 15184-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15184-1-AP (CPT1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CPT1A Polyclonal antibody (15184-1-AP)

    IHC staining of human liver cancer using 15184-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15184-1-AP (CPT1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CPT1A Polyclonal antibody (15184-1-AP)

    IHC staining of mouse brain using 15184-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15184-1-AP (CPT1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CPT1A Polyclonal antibody (15184-1-AP)

    IF Staining of HepG2 using 15184-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 15184-1-AP (CPT1A antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CPT1A Polyclonal antibody (15184-1-AP)

    IF Staining of HepG2 using 15184-1-AP

    Immunofluorescent analysis of -20°C Ethanol fixed HepG2 cells stained for CPT1A rabbit pAb (15184-1-AP) and KRT18 mouse mAb (66187-1-Ig) at dilution of 1:50, further stained with Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 15184-1-AP, and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) for 66187-1-Ig.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CPT1A Polyclonal antibody (15184-1-AP)

    IF Staining of HeLa using 15184-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CPT1A antibody (15184-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using CPT1A Polyclonal antibody (15184-1-AP)

    FC experiment of HeLa using 15184-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human CPT1A (15184-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inHeLa cells, A549 cells, HepG2 cells, MCF-7 cells, mouse liver tissue, rat liver tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15184-1-AP targets CPT1A in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, chicken, zebrafish, bovine, goat, yellow croaker, duck
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CPT1A fusion protein Ag7202 相同性解析による交差性が予測される生物種
Full Name carnitine palmitoyltransferase 1A (liver)
Calculated molecular weight 88 kDa
Observed molecular weight 86 kDa
GenBank accession numberBC000185
Gene symbol CPT1A
Gene ID (NCBI) 1374
RRIDAB_2084676
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CPT1A, also named CPT1, CPT1-L and L-CPTI, belongs to the carnitine/choline acetyltransferase family. It is Localized Chromosome 11q13.1-2. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CPT1A is an active form of related liver-type carnitine palmitoyltransferase I (PMID: 11001805). CPT1A deficiency presents as recurrent attacks of fasting hypoketotic hypoglycemia. (PMID: 15363638). This antibody can bind the close sequences genes.

Protocols

Product Specific Protocols
WB protocol for CPT1A antibody 15184-1-APDownload protocol
IHC protocol for CPT1A antibody 15184-1-APDownload protocol
IF protocol for CPT1A antibody 15184-1-APDownload protocol
IP protocol for CPT1A antibody 15184-1-APDownload protocol
FC protocol for CPT1A antibody 15184-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Res

Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation

Authors - Yunzi Mao
View Article
mouseWB

Nature

High-fat diet enhances stemness and tumorigenicity of intestinal progenitors.

Authors - Semir Beyaz
View Article
mouseWB,IF

Cell Metab

Quiescent Endothelial Cells Upregulate Fatty Acid β-Oxidation for Vasculoprotection via Redox Homeostasis.

Authors - Joanna Kalucka
View Article
mouseWB,FC

Cell

Mitochondrial Priming by CD28.

Authors - Ramon I Klein Geltink
  • KD Validated
View Article
mouseWB

Cell Metab

Targeting Erbin-mitochondria axis in platelets/megakaryocytes promotes B cell-mediated antitumor immunity

Authors - Zilong Zhang
View Article
mouseIHC

Cell Metab

SGLT2 Inhibition Mediates Protection from Diabetic Kidney Disease by Promoting Ketone Body-Induced mTORC1 Inhibition.

Authors - Issei Tomita
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Yuan (Verified Customer) (09-12-2024)

We suffered from poor images with CPT1 antibodies purchased from other companies. This antibody saved us. The top band is CPT1A at 80 kDa and the bottom one is GAPDH in the attached graph.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500 in 1% BSA/TBST
  • Cell Tissue Type: Human primary dermal fibroblast
CPT1A Antibody Western Blot validation (1:500 in 1% BSA/TBST dilution) in Human primary dermal fibroblast (Cat no:15184-1-AP)
FH

Tom (Verified Customer) (05-17-2021)

Antibody used at 1/1000 for 24 hours at 4 degrees. Non-specific bands were also present upon imaging.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HEK293
CPT1A Antibody Western Blot validation (1/1000 dilution) in HEK293 (Cat no:15184-1-AP)
FH

Linlin (Verified Customer) (05-08-2019)

This CPT1A antibody is ideal for precipitation of CPT1A in cell lysates and immunoblot analysis of CPT1A. Recommend.

  • Applications: Western Blot, Immunoprecipitation,
  • Primary Antibody Dilution: 2ug per pulldown; 1000x for immunoblot analysis
  • Cell Tissue Type: mice lung macrophages cell line
CPT1A Antibody Western Blot,Immunoprecipitation, validation (2ug per pulldown; 1000x for immunoblot analysis dilution) in mice lung macrophages cell line (Cat no:15184-1-AP)