Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis of A549 cells using 15184-1-AP
WB results of CPT1A antibody (1:1000) with shCPT1A-A549 and shCPT1A-HepG2 cells. shControl-A549 and shControl-HepG2 as positive control.
WB analysis using 15184-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15184-1-AP (CPT1A antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
WB analysis using 15184-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15184-1-AP (CPT1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 15184-1-AP
IP result of anti-CPT1A (IP:15184-1-AP, 4ug; Detection:15184-1-AP 1:1000) with HepG2 cells lysate 3000 ug.
IHC staining of human liver cancer using 15184-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15184-1-AP (CPT1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 15184-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15184-1-AP (CPT1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 15184-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15184-1-AP (CPT1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 15184-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15184-1-AP (CPT1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 15184-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 15184-1-AP (CPT1A antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 15184-1-AP
Immunofluorescent analysis of -20°C Ethanol fixed HepG2 cells stained for CPT1A rabbit pAb (15184-1-AP) and KRT18 mouse mAb (66187-1-Ig) at dilution of 1:50, further stained with Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 15184-1-AP, and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) for 66187-1-Ig.
IF Staining of HeLa using 15184-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CPT1A antibody (15184-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
IF Staining of HepG2 using 15184-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CPT1A antibody (15184-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).
FC experiment of HeLa using 15184-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human CPT1A (15184-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

CPT1A Polyclonal antibody

CPT1A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 15184-1-AP

Publications(373)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA

Synonyms

CPT1L, CPT-1A, CPT1, CPT I, Carnitine O-palmitoyltransferase I, liver isoform

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647 CoraLite®555

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (373)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HepG2 cells, A549 cells, HeLa cells, MCF-7 cells, mouse liver tissue, rat liver tissue, mouse spleen tissue
Positive IP detected in	HepG2 cells
Positive IHC detected in	human liver cancer tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, HepG2 cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:300-1:1200
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 18 publications below
WB	See 339 publications below
IHC	See 37 publications below
IF	See 24 publications below
IP	See 3 publications below
FC	See 5 publications below
CoIP	See 1 publications below
ChIP	See 1 publications below

Product Information

15184-1-AP targets CPT1A in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, chicken, zebrafish, bovine, goat, yellow croaker, duck
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CPT1A fusion protein Ag7202 相同性解析による交差性が予測される生物種
Full Name	carnitine palmitoyltransferase 1A (liver)
Calculated molecular weight	88 kDa
Observed molecular weight	86 kDa
GenBank accession number	BC000185
Gene Symbol	CPT1A
Gene ID (NCBI)	1374
RRID	AB_2084676
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P50416
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CPT1A, also named CPT1, CPT1-L and L-CPTI, belongs to the carnitine/choline acetyltransferase family. CPT1A gene is localized Chromosome 11q13.1-2. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CPT1A is an active form of related liver-type carnitine palmitoyltransferase I (PMID: 11001805). CPT1A deficiency presents as recurrent attacks of fasting hypoketotic hypoglycemia. (PMID: 15363638). This antibody can bind the close sequences genes.

Protocols

Product Specific Protocols
WB protocol for CPT1A antibody 15184-1-AP	Download protocol
IHC protocol for CPT1A antibody 15184-1-AP	Download protocol
IF protocol for CPT1A antibody 15184-1-AP	Download protocol
IP protocol for CPT1A antibody 15184-1-AP	Download protocol
FC protocol for CPT1A antibody 15184-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Cell Res Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation Authors - Yunzi Mao View Article
mouse	WB	Nature High-fat diet enhances stemness and tumorigenicity of intestinal progenitors. Authors - Semir Beyaz View Article
mouse	WB,IF	Cell Metab Quiescent Endothelial Cells Upregulate Fatty Acid β-Oxidation for Vasculoprotection via Redox Homeostasis. Authors - Joanna Kalucka View Article
mouse	WB,FC	Cell Mitochondrial Priming by CD28. Authors - Ramon I Klein Geltink KD Validated View Article
mouse	WB	Cell Metab Targeting Erbin-mitochondria axis in platelets/megakaryocytes promotes B cell-mediated antitumor immunity Authors - Zilong Zhang View Article
		Cell Metab Dynamic Cardiolipin Synthesis Is Required for CD8+ T Cell Immunity. Authors - Mauro Corrado View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of A549 cells using 15184-1-AP

WB analysis using 15184-1-AP

WB analysis using 15184-1-AP

IP experiment of HepG2 using 15184-1-AP

IHC staining of human liver cancer using 15184-1-AP

IHC staining of mouse brain using 15184-1-AP

IHC staining of human liver cancer using 15184-1-AP

IHC staining of mouse brain using 15184-1-AP

IF Staining of HepG2 using 15184-1-AP

IF Staining of HepG2 using 15184-1-AP

IF Staining of HeLa using 15184-1-AP

IF Staining of HepG2 using 15184-1-AP

FC experiment of HeLa using 15184-1-AP

CPT1A Polyclonal antibody

CPT1A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation

High-fat diet enhances stemness and tumorigenicity of intestinal progenitors.

Quiescent Endothelial Cells Upregulate Fatty Acid β-Oxidation for Vasculoprotection via Redox Homeostasis.

Mitochondrial Priming by CD28.

Targeting Erbin-mitochondria axis in platelets/megakaryocytes promotes B cell-mediated antitumor immunity

Dynamic Cardiolipin Synthesis Is Required for CD8+ T Cell Immunity.