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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®555-conjugated CPT1A Polyclonal antibody (CL555-15184)

    IF Staining of HepG2 using CL555-15184

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®555 CPT1A antibody (CL555-15184) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®555-conjugated CPT1A Polyclonal antibody (CL555-15184)

    FC experiment of HeLa using CL555-15184

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human CPT1A (CL555-15184) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  • Featured Product
  • KD/KO Validated

CoraLite®555-conjugated CPT1A Polyclonal antibody

CPT1A Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL555-15184

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

CPT1L, CPT-1A, CPT1, CPT I, Carnitine O-palmitoyltransferase I, liver isoform

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®555
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-15184 targets CPT1A in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CPT1A fusion protein Ag7202 相同性解析による交差性が予測される生物種
Full Name carnitine palmitoyltransferase 1A (liver)
Calculated molecular weight 88 kDa
Observed molecular weight 86 kDa
GenBank accession numberBC000185
Gene Symbol CPT1A
Gene ID (NCBI) 1374
RRIDAB_2919624
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP50416
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CPT1A, also named as CPT1, CPT1-L and L-CPTI, belongs to the carnitine/choline acetyltransferase family. It is Localized Chromosome 11q13.1-2. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CPT1A is an active forms of related liver-type carnitine palmitoyltransferase I. (PMID: 11001805). CPT1A deficiency presents as recurrent attacks of fasting hypoketotic hypoglycemia. (PMID: 15363638). This antibody can bind the close sequences genes.

Protocols

Product Specific Protocols
IF protocol for CL555 CPT1A antibody CL555-15184Download protocol
FC protocol for CL555 CPT1A antibody CL555-15184Download protocol
Standard Protocols
Click here to view our Standard Protocols