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CoraLite®555-conjugated CPT1A Polyclonal antibody
CPT1A Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC (Intra)
Conjugate
CoraLite®555 Fluorescent Dye
Cat no : CL555-15184
Synonyms
Validation Data Gallery
Tested Applications
| Positive FC (Intra) detected in | HeLa cells |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL555-15184 targets CPT1A in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CPT1A fusion protein Ag7202 相同性解析による交差性が予測される生物種 |
| Full Name | carnitine palmitoyltransferase 1A (liver) |
| Calculated molecular weight | 88 kDa |
| Observed molecular weight | 86 kDa |
| GenBank accession number | BC000185 |
| Gene symbol | CPT1A |
| Gene ID (NCBI) | 1374 |
| RRID | AB_2919624 |
| Conjugate | CoraLite®555 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 557 nm / 570 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CPT1A, also named as CPT1, CPT1-L and L-CPTI, belongs to the carnitine/choline acetyltransferase family. It is Localized Chromosome 11q13.1-2. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CPT1A is an active forms of related liver-type carnitine palmitoyltransferase I. (PMID: 11001805). CPT1A deficiency presents as recurrent attacks of fasting hypoketotic hypoglycemia. (PMID: 15363638). This antibody can bind the close sequences genes.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL555 CPT1A antibody CL555-15184 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
