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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CREB3L4 Polyclonal antibody (13630-1-AP)

    WB analysis using 13630-1-AP (same clone as 13630-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13630-1-AP (CREB3L4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13630-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using CREB3L4 Polyclonal antibody (13630-1-AP)

    WB analysis of HL-60 using 13630-1-AP (same clone as 13630-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 13630-1-AP (CREB3L4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13630-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CREB3L4 Polyclonal antibody (13630-1-AP)

    IF Staining of MCF-7 using 13630-1-AP (same clone as 13630-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 13630-1-AP (CREB3L4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13630-1-PBS in a different storage buffer formulation.

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CREB3L4 Polyclonal antibody, PBS Only

CREB3L4 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 13630-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13630-1-PBS targets CREB3L4 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CREB3L4 fusion protein Ag4548 相同性解析による交差性が予測される生物種
Full Name cAMP responsive element binding protein 3-like 4
Calculated molecular weight 395 aa, 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC038962
Gene Symbol CREB3L4
Gene ID (NCBI) 148327
RRIDAB_2276550
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TEY5
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CREB3L4 (cAMP responsive element binding protein 3-like 4), also named as AIBZIP, CREB4 and ATCE1, is a member of the CREB/ATF transcription factor family. CREB3L4 may play a role in the unfolded protein response, characterized by their regulation of gene expression through the cAMP-responsive element(PMID: 22829733). CREB3L4 is highly expressed in multiple human cancers, such as human breast carcinoma, prostate cancer(PMID: 32026099, PMID: 28338058). The molecular weight of CREB3L4 is 43 kDa and 55 kDa precursor was reported. Due to glycosylation, CREB3L4 can be observed with molecular weights of 35 and 41 kDa (PMID: 25412305, 15938716).