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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CRH/CRF Polyclonal antibody (26848-1-AP)

    WB analysis using 26848-1-AP (same clone as 26848-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 26848-1-AP (CRH/CRF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26848-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CRH/CRF Polyclonal antibody (26848-1-AP)

    IHC staining of human placenta using 26848-1-AP (same clone as 26848-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26848-1-AP (CRH/CRF antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26848-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CRH/CRF Polyclonal antibody (26848-1-AP)

    IHC staining of human placenta using 26848-1-AP (same clone as 26848-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26848-1-AP (CRH/CRF antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26848-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using CRH/CRF Polyclonal antibody (26848-1-AP)

    IF Staining of rat brain using 26848-1-AP (same clone as 26848-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CRH/CRF antibody (26848-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26848-1-PBS in a different storage buffer formulation.

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CRH/CRF Polyclonal antibody, PBS Only

CRH/CRF Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 26848-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

26848-1-PBS targets CRH/CRF in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRH/CRF fusion protein Ag25226 相同性解析による交差性が予測される生物種
Full Name CRH/CRF
Calculated molecular weight 21 kDa
Observed molecular weight27 kDa
GenBank accession numberBC011031
Gene Symbol CRH
Gene ID (NCBI) 1392
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP06850
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CRH, also called CRF or corticoliberin, is a peptide hormone and neurotransmitter involved in the stress response. Marked reduction in this protein has been observed in association with Alzheimer disease. In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. In the placenta it is a marker that determines the length of gestation and the timing of parturition and delivery.