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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CRMP1 Monoclonal antibody

CRMP1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Mouse, Rat, Rabbit, Pig, Chicken, Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2F4G5

Cat no : 68021-1-Ig

Synonyms

CRMP 1, CRMP1, DPYSL1, DRP 1, DRP1, ULIP 3, ULIP3, Unc 33 like phosphoprotein 3

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse cerebellum tissue using CRMP1 Monoclonal antibody (68021-1-Ig)

    WB analysis of mouse cerebellum using 68021-1-Ig

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 68021-1-Ig (CRMP1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using CRMP1 Monoclonal antibody (68021-1-Ig)

    WB analysis of pig brain using 68021-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 68021-1-Ig (CRMP1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rabbit brain tissue using CRMP1 Monoclonal antibody (68021-1-Ig)

    WB analysis of rabbit brain using 68021-1-Ig

    rabbit brain tissue were subjected to SDS PAGE followed by western blot with 68021-1-Ig (CRMP1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using CRMP1 Monoclonal antibody (68021-1-Ig)

    WB analysis of rat brain using 68021-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 68021-1-Ig (CRMP1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using CRMP1 Monoclonal antibody (68021-1-Ig)

    WB analysis of mouse brain using 68021-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 68021-1-Ig (CRMP1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of chicken brain tissue using CRMP1 Monoclonal antibody (68021-1-Ig)

    WB analysis of chicken brain using 68021-1-Ig

    chicken brain tissue were subjected to SDS PAGE followed by western blot with 68021-1-Ig (CRMP1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig cerebellum tissue using CRMP1 Monoclonal antibody (68021-1-Ig)

    WB analysis of pig cerebellum using 68021-1-Ig

    pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 68021-1-Ig (CRMP1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rabbit cerebellum tissue using CRMP1 Monoclonal antibody (68021-1-Ig)

    WB analysis of rabbit cerebellum using 68021-1-Ig

    rabbit cerebellum tissue were subjected to SDS PAGE followed by western blot with 68021-1-Ig (CRMP1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat cerebellum tissue using CRMP1 Monoclonal antibody (68021-1-Ig)

    WB analysis of rat cerebellum using 68021-1-Ig

    rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 68021-1-Ig (CRMP1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CRMP1 Monoclonal antibody (68021-1-Ig)

    IHC staining of mouse brain using 68021-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68021-1-Ig (CRMP1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CRMP1 Monoclonal antibody (68021-1-Ig)

    IHC staining of human lung cancer using 68021-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68021-1-Ig (CRMP1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CRMP1 Monoclonal antibody (68021-1-Ig)

    IHC staining of human lung cancer using 68021-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68021-1-Ig (CRMP1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CRMP1 Monoclonal antibody (68021-1-Ig)

    IHC staining of mouse brain using 68021-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68021-1-Ig (CRMP1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CRMP1 Monoclonal antibody (68021-1-Ig)

    IHC staining of mouse brain using 68021-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68021-1-Ig (CRMP1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CRMP1 Monoclonal antibody (68021-1-Ig)

    IHC staining of mouse brain using 68021-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68021-1-Ig (CRMP1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse cerebellum tissue, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, chicken brain tissue, pig cerebellum tissue, rabbit cerebellum tissue, rat cerebellum tissue
Positive IHC detected inmouse brain tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68021-1-Ig targets CRMP1 in WB, IHC, ELISA applications and shows reactivity with Mouse, Rat, Rabbit, Pig, Chicken, Human samples.

Tested Reactivity Mouse, Rat, Rabbit, Pig, Chicken, Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CRMP1 fusion protein Ag30090 相同性解析による交差性が予測される生物種
Full Name collapsin response mediator protein 1
Calculated molecular weight 62 kDa
Observed molecular weight62 kDa
GenBank accession numberBC000252
Gene symbol CRMP1
Gene ID (NCBI) 1400
RRIDAB_2918766
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CRMP1 (Collapsin response mediator protein 1) is also named as DPYSL1, ULIP3, DRP-1 and belongs to the collapsin response mediator protein family, which are a family of five cytoplasmic proteins predominantly expressed in the developing nervous system. CRMP1, the predicted 62 kDa protein and its 74 kDa isoform corresponding to its N-terminal splice variant already described for embryonic, neonatal, and adult brain of the rat are evidenced in the cortex of adult mouse (PMID:15834957). This protein is involved in invasion, and aggressiveness of prolactin pituitary tumors.

Protocols

Product Specific Protocols
WB protocol for CRMP1 antibody 68021-1-IgDownload protocol
IHC protocol for CRMP1 antibody 68021-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols