CoraLite® Plus 488-conjugated CRMP1 Monoclonal antibody
CRMP1 Monoclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat, pig, rabbit, chicken
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2F4G5
Cat no : CL488-68021
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | SH-SY5Y cells |
Positive FC (Intra) detected in | U2OS cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-68021 targets CRMP1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig, rabbit, chicken samples.
Tested Reactivity | human, mouse, rat, pig, rabbit, chicken |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | CRMP1 fusion protein Ag30090 相同性解析による交差性が予測される生物種 |
Full Name | collapsin response mediator protein 1 |
Calculated molecular weight | 62 kDa |
Observed molecular weight | 62 kDa |
GenBank accession number | BC000252 |
Gene symbol | CRMP1 |
Gene ID (NCBI) | 1400 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CRMP1 (Collapsin response mediator protein 1) is also named as DPYSL1, ULIP3, DRP-1 and belongs to the collapsin response mediator protein family, which are a family of five cytoplasmic proteins predominantly expressed in the developing nervous system. CRMP1, the predicted 62 kDa protein and its 74 kDa isoform corresponding to its N-terminal splice variant already described for embryonic, neonatal, and adult brain of the rat are evidenced in the cortex of adult mouse (PMID:15834957). This protein is involved in invasion, and aggressiveness of prolactin pituitary tumors.
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 CRMP1 antibody CL488-68021 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |