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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TORC2 Polyclonal antibody (12497-1-AP)

    WB analysis using 12497-1-AP (same clone as 12497-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12497-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using TORC2 Polyclonal antibody (12497-1-AP)

    WB analysis of mouse liver using 12497-1-AP (same clone as 12497-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12497-1-AP (CRTC2,TORC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12497-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using TORC2 Polyclonal antibody (12497-1-AP)

    WB analysis of mouse liver using 12497-1-AP (same clone as 12497-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12497-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using TORC2 Polyclonal antibody (12497-1-AP)

    WB analysis of HEK-293 using 12497-1-AP (same clone as 12497-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12497-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using TORC2 Polyclonal antibody (12497-1-AP)

    IHC staining of human gliomas using 12497-1-AP (same clone as 12497-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12497-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using TORC2 Polyclonal antibody (12497-1-AP)

    IHC staining of rat kidney using 12497-1-AP (same clone as 12497-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12497-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using TORC2 Polyclonal antibody (12497-1-AP)

    IHC staining of human skin cancer using 12497-1-AP (same clone as 12497-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12497-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using TORC2 Polyclonal antibody (12497-1-AP)

    IHC staining of human urothelial carcinoma using 12497-1-AP (same clone as 12497-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12497-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using TORC2 Polyclonal antibody (12497-1-AP)

    IHC staining of mouse lung using 12497-1-AP (same clone as 12497-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12497-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using TORC2 Polyclonal antibody (12497-1-AP)

    IHC staining of mouse kidney using 12497-1-AP (same clone as 12497-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12497-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TORC2 Polyclonal antibody (12497-1-AP)

    IF Staining of HepG2 using 12497-1-AP (same clone as 12497-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CRTC2,TORC2 antibody (12497-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 12497-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TORC2 Polyclonal antibody (12497-1-AP)

    IF Staining of HeLa using 12497-1-AP (same clone as 12497-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12497-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

TORC2 Polyclonal antibody, PBS Only

TORC2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 12497-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CRTC2,TORC2, CRTC2, Transducer of regulated cAMP response element-binding protein 2, Transducer of CREB protein 2, TORC 2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12497-1-PBS targets TORC2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TORC2 fusion protein Ag3167 相同性解析による交差性が予測される生物種
Full Name CREB regulated transcription coactivator 2
Calculated molecular weight 693 aa, 73 kDa
Observed molecular weight 73 kDa
GenBank accession numberBC053562
Gene Symbol TORC2
Gene ID (NCBI) 200186
RRIDAB_2260910
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ53ET0
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CRTC2, also named as TORC2, belongs to the TORC family. It is a transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. It acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. CRTC2 enhances the interaction of CREB1 with TAF4. Ir regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. CRTC2 regulates the expression of specific genes such as the steroidogenic gene, StAR. TORC2 was recently shown to be an important regulator of gluconeogenesis in the livers of mammals. It is one of the other key regulators of CRE-dependent MIE gene expression in NT2 cells. This regulation is linked to VIP-induced TORC2 dephosphorylation and translocation to the nucleus. (PMID: 19369332, 20504934 ) .