Mu Crystallin Polyclonal antibody

Name: Mu Crystallin Polyclonal antibody
Brand: Proteintech
SKU: 12495-1-AP

Mu Crystallin Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12495-1-AP

Synonyms

CRYM, NADP-regulated thyroid-hormone-binding protein, Ketimine reductase mu-crystallin, EC:1.5.1.25, DFNA40

Validation Data Gallery

Filter:

AllWBIHCIF-PFC (Intra)

WB analysis of Y79 using 12495-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 12495-1-AP (mu Crystallin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 12495-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 12495-1-AP (mu Crystallin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 12495-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 12495-1-AP (mu Crystallin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 12495-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 12495-1-AP (mu Crystallin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC staining of human gliomas using 12495-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12495-1-AP (mu Crystallin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 12495-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12495-1-AP (mu Crystallin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human gliomas using 12495-1-AP
Immunofluorescent analysis of (4% PFA) fixed human gliomas tissue using Mu Crystallin antibody (12495-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of human gliomas using 12495-1-AP
Immunofluorescent analysis of (4% PFA) fixed human gliomas tissue using Mu Crystallin antibody (12495-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
FC experiment of SH-SY5Y using 12495-1-AP
1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human Mu Crystallin (12495-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 12495-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Y79 cells, Jurkat cells, human kidney tissue, human heart tissue
Positive IHC detected in	human gliomas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human gliomas tissue
Positive FC (Intra) detected in	SH-SY5Y cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IHC	See 1 publications below
IF	See 2 publications below

Product Information

12495-1-AP targets Mu Crystallin in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Mu Crystallin fusion protein Ag3161 相同性解析による交差性が予測される生物種
Full Name	crystallin, mu
Calculated molecular weight	314 aa, 34 kDa
Observed molecular weight	34 kDa
GenBank accession number	BC018061
Gene symbol	Mu Crystallin
Gene ID (NCBI)	1428
RRID	AB_2084620
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

mu Crystallin(thiomorpholine-carboxylate dehydrogenase) is also named as THBP, CRYM, ketimine reductase and belongs to the ornithine cyclodeaminase family. This protein catalyzes the reduction of imine bonds in brain substrates that may include cystathionine ketimine (CysK) and lanthionine ketimine (LK). It is also involved in the regulation of the free intracellular concentration of triiodothyronine and access to its nuclear receptor.

Protocols

Product Specific Protocols
WB protocol for Mu Crystallin antibody 12495-1-AP	Download protocol
IHC protocol for Mu Crystallin antibody 12495-1-AP	Download protocol
IF protocol for Mu Crystallin antibody 12495-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Dev Cell Single-Cell Transcriptomic Analyses of the Developing Meninges Reveal Meningeal Fibroblast Diversity and Function. Authors - John DeSisto View Article
rat	WB	Endocr J Follicular thyroglobulin enhances gene expression necessary for thyroid hormone secretion. Authors - Yuko Ishido View Article
rat	WB,IHC,IF	Neuropathology Expression of CRYM in different rat organs during development and its decreased expression in degenerating pyramidal tracts in amyotrophic lateral sclerosis. Authors - Reiji Hommyo View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mu Crystallin Polyclonal antibody

Mu Crystallin Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Validation Data Gallery

WB analysis of Y79 using 12495-1-AP

WB analysis of Jurkat using 12495-1-AP

WB analysis of human kidney using 12495-1-AP

WB analysis of human heart using 12495-1-AP

IHC staining of human gliomas using 12495-1-AP

IHC staining of human gliomas using 12495-1-AP

IF Staining of human gliomas using 12495-1-AP

IF Staining of human gliomas using 12495-1-AP

FC experiment of SH-SY5Y using 12495-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Single-Cell Transcriptomic Analyses of the Developing Meninges Reveal Meningeal Fibroblast Diversity and Function.

Follicular thyroglobulin enhances gene expression necessary for thyroid hormone secretion.

Expression of CRYM in different rat organs during development and its decreased expression in degenerating pyramidal tracts in amyotrophic lateral sclerosis.

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Flow Cytometry Perm Buffer (10X)