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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated Mu Crystallin Polyclonal antibody

Mu Crystallin Polyclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-12495

Synonyms

CRYM, NADP-regulated thyroid-hormone-binding protein, Ketimine reductase mu-crystallin, EC:1.5.1.25, DFNA40

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human gliomas tissue using CoraLite®594-conjugated Mu Crystallin Polyclonal a (CL594-12495)

    IF Staining of human gliomas using CL594-12495

    Immunofluorescent analysis of (4% PFA) fixed human gliomas tissue using CoraLite®594 Mu Crystallin antibody (CL594-12495) at dilution of 1:200.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using CoraLite®594-conjugated Mu Crystallin Polyclonal a (CL594-12495)

    FC experiment of SH-SY5Y using CL594-12495

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human Mu Crystallin (CL594-12495) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF-P detected inhuman gliomas tissue
Positive FC (Intra) detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

CL594-12495 targets Mu Crystallin in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Mu Crystallin fusion protein Ag3161 相同性解析による交差性が予測される生物種
Full Name crystallin, mu
Calculated molecular weight 314 aa, 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC018061
Gene symbol Mu Crystallin
Gene ID (NCBI) 1428
RRIDAB_2919784
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

mu Crystallin(thiomorpholine-carboxylate dehydrogenase) is also named as THBP, CRYM, ketimine reductase and belongs to the ornithine cyclodeaminase family. This protein catalyzes the reduction of imine bonds in brain substrates that may include cystathionine ketimine (CysK) and lanthionine ketimine (LK). It is also involved in the regulation of the free intracellular concentration of triiodothyronine and access to its nuclear receptor.

Protocols

Product Specific Protocols
IF protocol for CL594 Mu Crystallin antibody CL594-12495Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Cell Stem Cell

SYF2 suppression mitigates neurodegeneration in models of diverse forms of ALS

Authors - Gabriel R Linares
View Article