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- KD/KO Validated
CSDE1 Polyclonal antibody
CSDE1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 13319-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, HEK-293 cells, HeLa cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:14000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 5 publications below |
Product Information
13319-1-AP targets CSDE1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CSDE1 fusion protein Ag4173 相同性解析による交差性が予測される生物種 |
| Full Name | cold shock domain containing E1, RNA-binding |
| Calculated molecular weight | 798 aa, 89 kDa |
| Observed molecular weight | 89 kDa |
| GenBank accession number | BC032446 |
| Gene symbol | CSDE1 |
| Gene ID (NCBI) | 7812 |
| RRID | AB_2084902 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CSDE1 (Cold shock domain-containing protein E1), also known as UNR (upstream of N-ras), is an RNA-binding protein involved in the regulation of messenger RNA stability and internal initiation of translation. It is required for internal initiation of translation of human rhinovirus RNA. UNR may be involved in translationally coupled mRNA turnover. Reduced expression of UNR impairs proliferation and differentiation of erythroid blasts. Recent study found that UNR acts as a positive or negative regulator of cell death, depending on the cell type, suggesting manipulation of UNR level may constitute a specific approach to sensitize cancer cells to anticancer treatments.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CSDE1 antibody 13319-1-AP | Download protocol |
| IHC protocol for CSDE1 antibody 13319-1-AP | Download protocol |
| IF protocol for CSDE1 antibody 13319-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Protoc Global analysis of RNA-binding protein dynamics by comparative and enhanced RNA interactome capture. | ||
Nat Commun Discovery of RNA-binding proteins and characterization of their dynamic responses by enhanced RNA interactome capture. | ||
Blood Ribosomal deficiencies in Diamond-Blackfan anemia impair translation of transcripts essential for differentiation of murine and human erythroblasts.
| ||
Viruses Upstream of N-Ras (Unr/CSDE1) Interacts with NCp7 and Gag, Modulating HIV-1 IRES-Mediated Translation Initiation
| ||
Nat Commun Systematic analysis of RNA-binding proteins identifies targetable therapeutic vulnerabilities in osteosarcoma | ||
Cell Rep The divergent effects of G3BP orthologs on human stress granule assembly imply a centric role for the core protein interaction network |
