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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CSDE1 Polyclonal antibody (13319-1-AP)

    WB analysis using 13319-1-AP (same clone as 13319-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13319-1-AP (CSDE1 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13319-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using CSDE1 Polyclonal antibody (13319-1-AP)

    WB analysis of A431 using 13319-1-AP (same clone as 13319-1-PBS)

    WB result of CSDE1 antibody (13319-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CSDE1 transfected A431 cells. This data was developed using the same antibody clone with 13319-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CSDE1 Polyclonal antibody (13319-1-AP)

    IHC staining of human liver cancer using 13319-1-AP (same clone as 13319-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13319-1-AP (CSDE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13319-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CSDE1 Polyclonal antibody (13319-1-AP)

    IF Staining of HeLa using 13319-1-AP (same clone as 13319-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CSDE1 antibody (13319-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 13319-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

CSDE1 Polyclonal antibody, PBS Only

CSDE1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 13319-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

UNR, NRU, N-ras upstream gene protein, KIAA0885, Cold shock domain-containing protein E1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13319-1-PBS targets CSDE1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CSDE1 fusion protein Ag4173 相同性解析による交差性が予測される生物種
Full Name cold shock domain containing E1, RNA-binding
Calculated molecular weight 798 aa, 89 kDa
Observed molecular weight 89 kDa
GenBank accession numberBC032446
Gene Symbol CSDE1
Gene ID (NCBI) 7812
RRIDAB_2084902
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75534
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CSDE1 (Cold shock domain-containing protein E1), also known as UNR (upstream of N-ras), is an RNA-binding protein involved in the regulation of messenger RNA stability and internal initiation of translation. It is required for internal initiation of translation of human rhinovirus RNA. UNR may be involved in translationally coupled mRNA turnover. Reduced expression of UNR impairs proliferation and differentiation of erythroid blasts. Recent study found that UNR acts as a positive or negative regulator of cell death, depending on the cell type, suggesting manipulation of UNR level may constitute a specific approach to sensitize cancer cells to anticancer treatments.