Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | A431 cells |
| Positive FC (Intra) detected in | A431 cells |
| Positive FC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-17720 targets CSK in IF/ICC, FC (Intra) applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CSK fusion protein Ag12103 相同性解析による交差性が予測される生物種 |
| Full Name | c-src tyrosine kinase |
| Calculated molecular weight | 450 aa, 50 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC106073 |
| Gene Symbol | CSK |
| Gene ID (NCBI) | 1445 |
| RRID | AB_3672680 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P41240 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CSK(Tyrosine-protein kinase CSK) is also named as C-Src kinase, protein-tyrosine kinase CYL and belongs to the protein kinase superfamily. Csk is a non-receptor tyrosine kinase with a molecular mass of 50 kDa. It contains the SH3 and SH2 domains in its N-terminus and a kinase domain in its C-terminus. It negatively regulates the kinase activity of SFKs and plays an important role in physiological functions via signaling pathways for cell proliferation, differentiation, adhesion, and migration(PMID:23548896). Csk may play a role in tumor suppression by inhibiting the oncogenic activity of SFKs. Indeed, overexpression of Csk can inhibit tumor growth of human colon cancer cells(PMID:23139636). This protein can be autophosphorylated(PMID:11181701).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CSK antibody CL488-17720 | Download protocol |
| FC protocol for CL Plus 488 CSK antibody CL488-17720 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |