CSNK2A1 Monoclonal antibody

CSNK2A1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rabbit

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1D5E8

Cat no : 68200-1-Ig

Synonyms

CKII, CK2α, CK2A1, CK2a, CK II alpha


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Tested Applications

Positive WB detected inLNCaP cells, rabbit heart tissue, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, MOLT-4 cells, K-562 cells, HL-60 cells
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:750-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68200-1-Ig targets CSNK2A1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, rabbit samples.

Tested Reactivity human, rabbit
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CSNK2A1 fusion protein Ag29062 相同性解析による交差性が予測される生物種
Full Name casein kinase 2, alpha 1 polypeptide
Calculated molecular weight 45 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC011668
Gene symbol CSNK2A1
Gene ID (NCBI) 1457
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CSNK2A1(Casein kinase II subunit alpha) is also named as CK2A1 and belongs to the CK2 subfamily. The CSNK2A1 gene contains 8 exons whose sequences comprise bases 102 to 824 of the coding region of the human casein kinase II alpha subunit. Casein kinase II is a ubiquitous protein-serine/threonine kinase in eukaryotic cells that catalyzes the phosphorylation of many protein substrates and has been regarded as a second-messenger-independent protein kinase. This enzyme is composed of an α subunit (37 to 44kDa) and a β subunit (24 to 28 kDa) and exists as an α2β2 tetramer (PMID:1448100). Casein kinase 2 has been found to be increased at protein level and up-regulated at the level of enzyme activity in the majority of cancers. However, elevated levels of casein kinase 2 are present in certain normal organs such as brain and testes.

Protocols

Product Specific Protocols
WB protocol for CSNK2A1 antibody 68200-1-IgDownload protocol
IF protocol for CSNK2A1 antibody 68200-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

F1000Res

A guide to selecting high-performing antibodies for CSNK2A1 (UniProt ID: P68400) for use in western blot, immunoprecipitation and immunofluorescence

Authors - Riham Ayoubi
  • KO Validated