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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CSNK2A1 Monoclonal antibody, PBS Only

CSNK2A1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rabbit

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D5E8

Cat no : 68200-1-PBS

Synonyms

CKII, CK2α, CK2A1, CK2a, CK II alpha

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CSNK2A1 Monoclonal antibody (68200-1-Ig)

    WB analysis using 68200-1-Ig (same clone as 68200-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68200-1-Ig (CSNK2A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68200-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rabbit heart tissue using CSNK2A1 Monoclonal antibody (68200-1-Ig)

    WB analysis of rabbit heart using 68200-1-Ig (same clone as 68200-1-PBS)

    rabbit heart tissue were subjected to SDS PAGE followed by western blot with 68200-1-Ig (CSNK2A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68200-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CSNK2A1 Monoclonal antibody (68200-1-Ig)

    IF Staining of A431 using 68200-1-Ig (same clone as 68200-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CSNK2A1 antibody (68200-1-Ig, Clone: 1D5E8 ) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68200-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68200-1-PBS targets CSNK2A1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, rabbit samples.

Tested Reactivity human, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CSNK2A1 fusion protein Ag29062 相同性解析による交差性が予測される生物種
Full Name casein kinase 2, alpha 1 polypeptide
Calculated molecular weight 45 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC011668
Gene symbol CSNK2A1
Gene ID (NCBI) 1457
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CSNK2A1(Casein kinase II subunit alpha) is also named as CK2A1 and belongs to the CK2 subfamily. The CSNK2A1 gene contains 8 exons whose sequences comprise bases 102 to 824 of the coding region of the human casein kinase II alpha subunit. Casein kinase II is a ubiquitous protein-serine/threonine kinase in eukaryotic cells that catalyzes the phosphorylation of many protein substrates and has been regarded as a second-messenger-independent protein kinase. This enzyme is composed of an α subunit (37 to 44kDa) and a β subunit (24 to 28 kDa) and exists as an α2β2 tetramer (PMID:1448100). Casein kinase 2 has been found to be increased at protein level and up-regulated at the level of enzyme activity in the majority of cancers. However, elevated levels of casein kinase 2 are present in certain normal organs such as brain and testes.