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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CSNK2A1 Recombinant antibody, PBS Only

CSNK2A1 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240862A7

Cat no : 83805-4-PBS

Synonyms

CKII, CK2α, CK2A1, CK2a, CK II alpha

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CSNK2A1 Recombinant antibody (83805-4-RR)

    WB analysis using 83805-4-RR (same clone as 83805-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83805-4-RR (CSNK2A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83805-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CSNK2A1 Recombinant antibody (83805-4-RR)

    IF Staining of HeLa using 83805-4-RR (same clone as 83805-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CSNK2A1 antibody (83805-4-RR, Clone: 240862A7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83805-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CSNK2A1 Recombinant antibody (83805-4-RR)

    IF Staining of HeLa using 83805-4-RR (same clone as 83805-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CSNK2A1 antibody (83805-4-RR, Clone: 240862A7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83805-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Jurkat cells using CSNK2A1 Recombinant antibody (83805-4-RR)

    FC experiment of Jurkat using 83805-4-RR (same clone as 83805-4-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug CSNK2A1 Recombinant antibody (83805-4-RR, Clone:240862A7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83805-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83805-4-RR

    Affinity and Kinetic Characterization of 83805-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83805-4-RR against Human CSNK2A1 were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83805-4-PBS is CSNK2A1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CSNK2A1 fusion protein Ag1452 相同性解析による交差性が予測される生物種
Full Name casein kinase 2, alpha 1 polypeptide
Calculated molecular weight 45 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC011668
Gene symbol CSNK2A1
Gene ID (NCBI) 1457
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CSNK2A1(Casein kinase II subunit alpha) is also named as CK2A1 and belongs to the CK2 subfamily. The CSNK2A1 gene contains 8 exons whose sequences comprise bases 102 to 824 of the coding region of the human casein kinase II alpha subunit. Casein kinase II is a ubiquitous protein-serine/threonine kinase in eukaryotic cells that catalyzes the phosphorylation of many protein substrates and has been regarded as a second-messenger-independent protein kinase. This enzyme is composed of an α subunit (37 to 44kDa) and a β subunit (24 to 28 kDa) and exists as an α2β2 tetramer (PMID:1448100). Casein kinase 2 has been found to be increased at protein level and up-regulated at the level of enzyme activity in the majority of cancers. However, elevated levels of casein kinase 2 are present in certain normal organs such as brain and testes.