CSNK2A1 Recombinant antibody
CSNK2A1 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF/ICC, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
240862A7
Cat no : 83805-4-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293T cells, Raji cells, SH-SY5Y cells, U-251 cells, A431 cells, C6 cells, NIH/3T3 cells |
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | Jurkat cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of 83805-4-RR is CSNK2A1 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | CSNK2A1 fusion protein Ag1452 相同性解析による交差性が予測される生物種 |
Full Name | casein kinase 2, alpha 1 polypeptide |
Calculated molecular weight | 45 kDa |
Observed molecular weight | 40-45 kDa |
GenBank accession number | BC011668 |
Gene symbol | CSNK2A1 |
Gene ID (NCBI) | 1457 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CSNK2A1(Casein kinase II subunit alpha) is also named as CK2A1 and belongs to the CK2 subfamily. The CSNK2A1 gene contains 8 exons whose sequences comprise bases 102 to 824 of the coding region of the human casein kinase II alpha subunit. Casein kinase II is a ubiquitous protein-serine/threonine kinase in eukaryotic cells that catalyzes the phosphorylation of many protein substrates and has been regarded as a second-messenger-independent protein kinase. This enzyme is composed of an α subunit (37 to 44kDa) and a β subunit (24 to 28 kDa) and exists as an α2β2 tetramer (PMID:1448100). Casein kinase 2 has been found to be increased at protein level and up-regulated at the level of enzyme activity in the majority of cancers. However, elevated levels of casein kinase 2 are present in certain normal organs such as brain and testes.
Protocols
Product Specific Protocols | |
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WB protocol for CSNK2A1 antibody 83805-4-RR | Download protocol |
IF protocol for CSNK2A1 antibody 83805-4-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |