Validation Data Gallery

Filter: AllWBIF/ICC

WB analysis of Jurkat using 11931-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 11931-1-AP (CSPP1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 11931-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 11931-1-AP (CSPP1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11931-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11931-1-AP (CSPP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 11931-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 11931-1-AP (CSPP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11931-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11931-1-AP (CSPP1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of rat testis using 11931-1-AP
rat testis tissue were subjected to SDS PAGE followed by western blot with 11931-1-AP (CSPP1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
WB analysis of HEK-293 using 11931-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11931-1-AP (CSPP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IF Staining of HeLa using 11931-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CSPP1 antibody (11931-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

CSPP1 Polyclonal antibody

CSPP1 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 11931-1-AP

Publications(12)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

CSPP, Centrosome and spindle pole-associated protein 1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (12)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Jurkat cells, HEK-293 cells, HeLa cells, mouse testis tissue, rat testis tissue
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 7 publications below
IHC	See 1 publications below
IF	See 9 publications below
IP	See 1 publications below

Product Information

11931-1-AP targets CSPP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CSPP1 fusion protein Ag2521 相同性解析による交差性が予測される生物種
Full Name	centrosome and spindle pole associated protein 1
Calculated molecular weight	141 kDa
Observed molecular weight	141 kDa
GenBank accession number	BC029445
Gene Symbol	CSPP1
Gene ID (NCBI)	79848
RRID	AB_2087897
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q1MSJ5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CSPP1 interacts with centrosomes and microtubules and plays a role in cell-cycle progression and spindle organization. CSPP1 is required for their common C-terminal domain to interact with Nephrocystin 8 (NPHP8/RPGRIP1L) and to form a ternary complex with NPHP8 and NPHP4, and for ciliogenesis in hTERT-RPE1 cells in vitro and are expressed in ciliated renal, retinal, and respiratory cells in vivo

Protocols

Product Specific Protocols
WB protocol for CSPP1 antibody 11931-1-AP	Download protocol
IF protocol for CSPP1 antibody 11931-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF	Am J Hum Genet Mutations in CSPP1 Cause Primary Cilia Abnormalities and Joubert Syndrome with or without Jeune Asphyxiating Thoracic Dystrophy. Authors - Karina Tuz View Article
human	WB,IF	Am J Hum Genet Mutations in CSPP1 Lead to Classical Joubert Syndrome. Authors - Naiara Akizu View Article
human	WB	Cell Rep A CEP104-CSPP1 Complex Is Required for Formation of Primary Cilia Competent in Hedgehog Signaling. Authors - Kari-Anne M Frikstad KO Validated View Article
human	WB	EMBO Rep CK1-mediated phosphorylation of FAM110A promotes its interaction with mitotic spindle and controls chromosomal alignment. Authors - Cecilia Aquino Perez KD Validated View Article
human	IF	PLoS Biol The ciliopathy protein CCDC66 controls mitotic progression and cytokinesis by promoting microtubule nucleation and organization. Authors - Umut Batman View Article
human	IHC,IF	Br J Cancer Nuclear CSPP1 expression defined subtypes of basal-like breast cancer. Authors - J Sternemalm KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of Jurkat using 11931-1-AP

WB analysis of mouse testis using 11931-1-AP

WB analysis of HEK-293 using 11931-1-AP

WB analysis of Jurkat using 11931-1-AP

WB analysis of HeLa using 11931-1-AP

WB analysis of rat testis using 11931-1-AP

WB analysis of HEK-293 using 11931-1-AP

IF Staining of HeLa using 11931-1-AP

CSPP1 Polyclonal antibody

CSPP1 Polyclonal Antibody for WB, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Mutations in CSPP1 Cause Primary Cilia Abnormalities and Joubert Syndrome with or without Jeune Asphyxiating Thoracic Dystrophy.

Mutations in CSPP1 Lead to Classical Joubert Syndrome.

A CEP104-CSPP1 Complex Is Required for Formation of Primary Cilia Competent in Hedgehog Signaling.

CK1-mediated phosphorylation of FAM110A promotes its interaction with mitotic spindle and controls chromosomal alignment.

The ciliopathy protein CCDC66 controls mitotic progression and cytokinesis by promoting microtubule nucleation and organization.

Nuclear CSPP1 expression defined subtypes of basal-like breast cancer.