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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CSPP1 Polyclonal antibody

CSPP1 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11931

Synonyms

CSPP, CSPP1, FLJ22490, FLJ38886

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated CSPP1 Polyclonal ant (CL488-11931)

    IF Staining of HeLa using CL488-11931

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 CSPP1 antibody (CL488-11931) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-11931 targets CSPP1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CSPP1 fusion protein Ag2521 相同性解析による交差性が予測される生物種
Full Name centrosome and spindle pole associated protein 1
Calculated molecular weight 141 kDa
Observed molecular weight 141 kDa
GenBank accession numberBC029445
Gene symbol CSPP1
Gene ID (NCBI) 79848
RRIDAB_2919043
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CSPP1 interacts with centrosomes and microtubules and plays a role in cell-cycle progression and spindle organization. CSPP1 is required for their common C-terminal domain to interact with Nephrocystin 8 (NPHP8/RPGRIP1L) and to form a ternary complex with NPHP8 and NPHP4, and for ciliogenesis in hTERT-RPE1 cells in vitro and are expressed in ciliated renal, retinal, and respiratory cells in vivo

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CSPP1 antibody CL488-11931Download protocol
Standard Protocols
Click here to view our Standard Protocols