Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of human saliva using 10823-1-AP
human saliva were subjected to SDS PAGE followed by western blot with 10823-1-AP (Cystatin B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 10823-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 10823-1-AP (Cystatin B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10823-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 10823-1-AP (Cystatin B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 10823-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 10823-1-AP (Cystatin B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP experiment of MCF-7 using 10823-1-AP
IP result of anti-Cystatin B (IP:10823-1-AP, 4ug; Detection:10823-1-AP 1:4000) with MCF-7 cells lysate 1320ug.
IHC staining of human colon using 10823-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 10823-1-AP (Cystatin B antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach using 10823-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10823-1-AP (Cystatin B antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 10823-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Cystatin B antibody (10823-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of HepG2 using 10823-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Cystatin B antibody (10823-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Featured Product
KD/KO Validated

Cystatin B Polyclonal antibody

Cystatin B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10823-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

CSTB, EPM1, Cystatin-B, CST6, CPI-B

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	human saliva tissue, MCF-7 cells, HepG2 cells, human saliva
Positive IP detected in	MCF-7 cells
Positive IHC detected in	human colon tissue, human stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells, HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 1 publications below
IF	See 1 publications below

Product Information

10823-1-AP targets Cystatin B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Cystatin B fusion protein Ag1262 相同性解析による交差性が予測される生物種
Full Name	cystatin B (stefin B)
Calculated molecular weight	11 kDa
Observed molecular weight	11 kDa
GenBank accession number	BC003370
Gene Symbol	Cystatin B
Gene ID (NCBI)	1476
RRID	AB_2086100
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P04080
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Cystatin B (CSTB), a member of the cystatin superfamily protein, is a stefin that functions as an intracellular thiol protease inhibitor and has been thought to play a role in protecting against the proteases leaking from lysosomes. CSTB plays various functions in a variety of diseases, including epithelial ovarian cancer, colon cancer, and myoclonus epilepsy. Evidence indicates that mutations in CSTB are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). One type of mutation responsible for EPM1 is the expansion in the promoter region of this gene of a CCCCGCCCCGCG repeat from 2-3 copies to 30-78 copies.

Protocols

Product Specific Protocols
WB protocol for Cystatin B antibody 10823-1-AP	Download protocol
IHC protocol for Cystatin B antibody 10823-1-AP	Download protocol
IF protocol for Cystatin B antibody 10823-1-AP	Download protocol
IP protocol for Cystatin B antibody 10823-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Matrix Biol Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility. Authors - Emna Ouni View Article
human	WB	Naunyn Schmiedebergs Arch Pharmacol Stefin B alleviates the gouty arthritis in mice by inducing the M2 polarization of macrophages Authors - Shishui Lin KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of human saliva using 10823-1-AP

WB analysis of MCF-7 using 10823-1-AP

WB analysis of HepG2 using 10823-1-AP

WB analysis of MCF-7 using 10823-1-AP

IP experiment of MCF-7 using 10823-1-AP

IHC staining of human colon using 10823-1-AP

IHC staining of human stomach using 10823-1-AP

IF Staining of MCF-7 using 10823-1-AP

IF Staining of HepG2 using 10823-1-AP