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  • Featured Product
  • KD/KO Validated

Cystatin B Polyclonal antibody

Cystatin B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10823-1-AP

Synonyms

CSTB, EPM1, Cystatin-B, CST6, CPI-B

Validation Data Gallery

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  • Western Blot (WB) analysis of human saliva using Cystatin B Polyclonal antibody (10823-1-AP)

    WB analysis of human saliva using 10823-1-AP

    human saliva were subjected to SDS PAGE followed by western blot with 10823-1-AP (Cystatin B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using Cystatin B Polyclonal antibody (10823-1-AP)

    WB analysis of MCF-7 using 10823-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 10823-1-AP (Cystatin B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using Cystatin B Polyclonal antibody (10823-1-AP)

    WB analysis of HepG2 using 10823-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10823-1-AP (Cystatin B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using Cystatin B Polyclonal antibody (10823-1-AP)

    WB analysis of MCF-7 using 10823-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 10823-1-AP (Cystatin B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using Cystatin B Polyclonal antibody (10823-1-AP)

    IP experiment of MCF-7 using 10823-1-AP

    IP result of anti-Cystatin B (IP:10823-1-AP, 4ug; Detection:10823-1-AP 1:4000) with MCF-7 cells lysate 1320ug.

  • Immunohistochemistry (IHC) staining of human colon tissue using Cystatin B Polyclonal antibody (10823-1-AP)

    IHC staining of human colon using 10823-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 10823-1-AP (Cystatin B antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using Cystatin B Polyclonal antibody (10823-1-AP)

    IHC staining of human stomach using 10823-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10823-1-AP (Cystatin B antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Cystatin B Polyclonal antibody (10823-1-AP)

    IF Staining of MCF-7 using 10823-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Cystatin B antibody (10823-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Cystatin B Polyclonal antibody (10823-1-AP)

    IF Staining of HepG2 using 10823-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Cystatin B antibody (10823-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inhuman saliva tissue, MCF-7 cells, HepG2 cells, human saliva
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman colon tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10823-1-AP targets Cystatin B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cystatin B fusion protein Ag1262 相同性解析による交差性が予測される生物種
Full Name cystatin B (stefin B)
Calculated molecular weight 11 kDa
Observed molecular weight 11 kDa
GenBank accession numberBC003370
Gene symbol Cystatin B
Gene ID (NCBI) 1476
RRIDAB_2086100
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cystatin B (CSTB), a member of the cystatin superfamily protein, is a stefin that functions as an intracellular thiol protease inhibitor and has been thought to play a role in protecting against the proteases leaking from lysosomes. CSTB plays various functions in a variety of diseases, including epithelial ovarian cancer, colon cancer, and myoclonus epilepsy. Evidence indicates that mutations in CSTB are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). One type of mutation responsible for EPM1 is the expansion in the promoter region of this gene of a CCCCGCCCCGCG repeat from 2-3 copies to 30-78 copies.

Protocols

Product Specific Protocols
WB protocol for Cystatin B antibody 10823-1-APDownload protocol
IHC protocol for Cystatin B antibody 10823-1-APDownload protocol
IF protocol for Cystatin B antibody 10823-1-APDownload protocol
IP protocol for Cystatin B antibody 10823-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Matrix Biol

Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.

Authors - Emna Ouni
View Article
humanWB

Naunyn Schmiedebergs Arch Pharmacol

Stefin B alleviates the gouty arthritis in mice by inducing the M2 polarization of macrophages

Authors - Shishui Lin
  • KD Validated
View Article