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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Cystatin B Polyclonal antibody

Cystatin B Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10823

Synonyms

CSTB, EPM1, Cystatin-B, CST6, CPI-B

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite® Plus 488-conjugated Cystatin B Polyclona (CL488-10823)

    IF Staining of A431 using CL488-10823

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 Cystatin B antibody (CL488-10823) at dilution of 1:200, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite® Plus 488-conjugated Cystatin B Polyclona (CL488-10823)

    FC experiment of MCF-7 using CL488-10823

    1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Cystatin B Polyclonal Antibody (CL488-10823)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-10823 targets Cystatin B in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cystatin B fusion protein Ag1262 相同性解析による交差性が予測される生物種
Full Name cystatin B (stefin B)
Calculated molecular weight 11 kDa
Observed molecular weight 11 kDa
GenBank accession numberBC003370
Gene symbol Cystatin B
Gene ID (NCBI) 1476
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen Affinity Purified
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cystatin B (CSTB), a member of the cystatin superfamily protein, is a stefin that functions as an intracellular thiol protease inhibitor and has been thought to play a role in protecting against the proteases leaking from lysosomes. CSTB plays various functions in a variety of diseases, including epithelial ovarian cancer, colon cancer, and myoclonus epilepsy. Evidence indicates that mutations in CSTB are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). One type of mutation responsible for EPM1 is the expansion in the promoter region of this gene of a CCCCGCCCCGCG repeat from 2-3 copies to 30-78 copies.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Cystatin B antibody CL488-10823Download protocol
Standard Protocols
Click here to view our Standard Protocols