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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated CTBP1 Monoclonal antibody (CL594-66995)

    IF Staining of HepG2 using CL594-66995

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 CTBP1 antibody (CL594-66995, Clone: 1C4B2 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite®594-conjugated CTBP1 Monoclonal antibody (CL594-66995)

    FC experiment of HepG2 using CL594-66995

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human CTBP1 (CL594-66995, Clone:1C4B2) (red), or 0.2 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

CoraLite®594-conjugated CTBP1 Monoclonal antibody

CTBP1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-66995
Clone No.1C4B2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

EC:1.1.1.-, C-terminal-binding protein 1, CTBP, BARS, 1C4B2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66995 targets CTBP1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CTBP1 fusion protein Ag28567 相同性解析による交差性が予測される生物種
Full Name C-terminal binding protein 1
Calculated molecular weight 48 kDa
Observed molecular weight 45-48 kDa
GenBank accession numberBC011655
Gene Symbol CTBP1
Gene ID (NCBI) 1487
RRIDAB_2883636
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13363
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CTBP1, also named as C-terminal-binding protein 1, is a 440 amino acid protein, which belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. CTBP1 is a cellular phosphoprotein that associates with various proteins and functions as a corepressor of transcription. CTBP1 and the related protein CTBP2 are characterized as C-terminal binding protein of adenovirus E1A, and they preferentially associate with the E1A via a 5-amino acid motif, PLDLS, to repress E1A induced oncogenesis and cellular transformation. CTBP1 is expressed from embryo to adult, but CTBP2 is mainly expressed during embryogenesis.

Protocols

Product Specific Protocols
IF protocol for CL594 CTBP1 antibody CL594-66995Download protocol
FC protocol for CL594 CTBP1 antibody CL594-66995Download protocol
Standard Protocols
Click here to view our Standard Protocols