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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CTLA-4/CD152 Rabbit Recombinant Antibod (98078-1-RR)

    FC experiment of human PBMCs using 98078-1-RR (same clone as 98078-1-PBS)

    1x10^6 PHA stimulated human PBMCs were surface stained with 0.25 ug Anti-Human CTLA4 Rabbit Recombinant Antibody (98078-1-RR, Clone:240793E8) , or 1x10^6 unstimulated human PBMCs were surface stained with 0.25 ug Anti-Human CTLA4 Rabbit Recombinant Antibody (98078-1-RR, Clone:240793E8) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Human CD3. Cells were not fixed. This data was developed using the same antibody clone with 98078-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CTLA-4/CD152 Rabbit Recombinant Antibod (98078-1-RR)

    FC experiment of human PBMCs using 98078-1-RR (same clone as 98078-1-PBS)

    1x10^6 PHA stimulated human PBMCs were surface stained with 0.25 ug Anti-Human CTLA4 Rabbit Recombinant Antibody (98078-1-RR, Clone:240793E8)(red), or Isotype Control(blue), or 1x10^6 unstimulated human PBMCs were surface stained with 0.25 ug Anti-Human CTLA4 Rabbit Recombinant Antibody (98078-1-RR, Clone:240793E8)(black) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) . Cells were not fixed. This data was developed using the same antibody clone with 98078-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98078-1-RR

    Affinity and Kinetic Characterization of 98078-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98078-1-RR against Human CTLA4/CD152 were performed. The affinity constant is 60.2 pM.

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Anti-Human CTLA-4/CD152 Rabbit Recombinant Antibody, PBS Only

CTLA-4/CD152 Uni-rAbTM Recombinant Antibody for FC
Cat No. 98078-1-PBS
Clone No.240793E8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CTLA4, Cytotoxic T-lymphocyte protein 4, CTLA 4, CELIAC3, CD152

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

98078-1-PBS targets CTLA-4/CD152 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name cytotoxic T-lymphocyte-associated protein 4
Calculated molecular weight 223 aa, 25 kDa
GenBank accession numberBC070162
Gene Symbol CTLA4
Gene ID (NCBI) 1493
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP16410
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.