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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CTLA-4/CD152 Rabbit Recombinant Antibody

CTLA-4/CD152 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240793E8

Cat no : 98078-1-RR

Synonyms

CTLA4, Cytotoxic T-lymphocyte protein 4, CTLA 4, CELIAC3, CD152

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CTLA-4/CD152 Rabbit Recombinant Antibod (98078-1-RR)

    FC experiment of human PBMCs using 98078-1-RR

    1x10^6 PHA stimulated human PBMCs were surface stained with 0.25 ug Anti-Human CTLA4 Rabbit Recombinant Antibody (98078-1-RR, Clone:240793E8) , or 1x10^6 unstimulated human PBMCs were surface stained with 0.25 ug Anti-Human CTLA4 Rabbit Recombinant Antibody (98078-1-RR, Clone:240793E8) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Human CD3. Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CTLA-4/CD152 Rabbit Recombinant Antibod (98078-1-RR)

    FC experiment of human PBMCs using 98078-1-RR

    1x10^6 PHA stimulated human PBMCs were surface stained with 0.25 ug Anti-Human CTLA4 Rabbit Recombinant Antibody (98078-1-RR, Clone:240793E8)(red), or Isotype Control(blue), or 1x10^6 unstimulated human PBMCs were surface stained with 0.25 ug Anti-Human CTLA4 Rabbit Recombinant Antibody (98078-1-RR, Clone:240793E8)(black) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) . Cells were not fixed.

  • Affinity and Kinetic Characterization of 98078-1-RR

    Affinity and Kinetic Characterization of 98078-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98078-1-RR against Human CTLA4/CD152 were performed. The affinity constant is 60.2 pM.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98078-1-RR is CTLA-4/CD152 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name cytotoxic T-lymphocyte-associated protein 4
Calculated molecular weight 223 aa, 25 kDa
GenBank accession numberBC070162
Gene symbol CTLA4
Gene ID (NCBI) 1493
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Protocols

Product Specific Protocols
FC protocol for CTLA-4/CD152 antibody 98078-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols