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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CUEDC2 Polyclonal antibody (20123-1-AP)

    WB analysis using 20123-1-AP (same clone as 20123-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20123-1-AP (CUEDC2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20123-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using CUEDC2 Polyclonal antibody (20123-1-AP)

    WB analysis of human brain using 20123-1-AP (same clone as 20123-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 20123-1-AP (CUEDC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20123-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human kidney tissue using CUEDC2 Polyclonal antibody (20123-1-AP)

    WB analysis of human kidney using 20123-1-AP (same clone as 20123-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 20123-1-AP (CUEDC2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20123-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using CUEDC2 Polyclonal antibody (20123-1-AP)

    WB analysis of mouse kidney using 20123-1-AP (same clone as 20123-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20123-1-AP (CUEDC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20123-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using CUEDC2 Polyclonal antibody (20123-1-AP)

    IP experiment of mouse brain using 20123-1-AP (same clone as 20123-1-PBS)

    IP result of anti-CUEDC2 (IP:20123-1-AP, 4ug; Detection:20123-1-AP 1:500) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 20123-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using CUEDC2 Polyclonal antibody (20123-1-AP)

    IHC staining of human ovary tumor using 20123-1-AP (same clone as 20123-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20123-1-AP (CUEDC2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20123-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using CUEDC2 Polyclonal antibody (20123-1-AP)

    IHC staining of human ovary tumor using 20123-1-AP (same clone as 20123-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20123-1-AP (CUEDC2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20123-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using CUEDC2 Polyclonal antibody (20123-1-AP)

    IHC staining of human malignant melanoma using 20123-1-AP (same clone as 20123-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 20123-1-AP (CUEDC2 antibody) at dilution of 1:800 (under 40x lens).. This data was developed using the same antibody clone with 20123-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using CUEDC2 Polyclonal antibody (20123-1-AP)

    IHC staining of human malignant melanoma using 20123-1-AP (same clone as 20123-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 20123-1-AP (CUEDC2 antibody) at dilution of 1:800 (under 40x lens).. This data was developed using the same antibody clone with 20123-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CUEDC2 Polyclonal antibody (20123-1-AP)

    IHC staining of human breast cancer using 20123-1-AP (same clone as 20123-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20123-1-AP (CUEDC2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20123-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CUEDC2 Polyclonal antibody (20123-1-AP)

    IHC staining of human breast cancer using 20123-1-AP (same clone as 20123-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20123-1-AP (CUEDC2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20123-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CUEDC2 Polyclonal antibody (20123-1-AP)

    IF Staining of HeLa using 20123-1-AP (same clone as 20123-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CUEDC2 antibody (20123-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 20123-1-PBS in a different storage buffer formulation.

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CUEDC2 Polyclonal antibody, PBS Only

CUEDC2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 20123-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20123-1-PBS targets CUEDC2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CUEDC2 fusion protein Ag13847 相同性解析による交差性が予測される生物種
Full Name CUE domain containing 2
Calculated molecular weight 287 aa, 32 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC000262
Gene Symbol CUEDC2
Gene ID (NCBI) 79004
RRIDAB_10638318
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H467
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CUE domain-containing 2 (CUEDC2) is a ubiquitin-binding motif-containing protein involved in the regulation of the cell cycle, inflammation, and tumorigenesis. It is ubiquitously expressed in human tissues and organs, not only highly expressed in the normal brain, heart and testis, but also some kinds of cancers, such as breast, ovarian and kidney cancers. CUEDC2 could act as an adaptor protein to target IκB kinase (IKK) for dephosphorylation and inactivation by recruiting protein phosphatase (PP1), and thus repressed activation of the transcription factor NF-κB. Moreover, CUEDC2, is a key factor in endocrine resistance in breast cancer.