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CUL2 Monoclonal antibody
CUL2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Rat and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
CloneNo.
1A6G9
Cat no : 67175-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | MCF-7 cells, HeLa cells, HEK-293 cells, PC-3 cells, HSC-T6 cells, Raji cells |
Positive IHC detected in | human breast cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 1 publications below |
IF | See 1 publications below |
IP | See 1 publications below |
Product Information
67175-1-Ig targets CUL2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, Rat samples.
Tested Reactivity | Human, Rat |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | CUL2 fusion protein Ag28654 相同性解析による交差性が予測される生物種 |
Full Name | cullin 2 |
Calculated molecular weight | 87 kDa |
Observed molecular weight | 85-90 kDa |
GenBank accession number | BC009591 |
Gene symbol | CUL2 |
Gene ID (NCBI) | 8453 |
RRID | AB_2882471 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Humans express seven cullin proteins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.
Protocols
Product Specific Protocols | |
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WB protocol for CUL2 antibody 67175-1-Ig | Download protocol |
IHC protocol for CUL2 antibody 67175-1-Ig | Download protocol |
IF protocol for CUL2 antibody 67175-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
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