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CUL2 Monoclonal antibody

CUL2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1A6G9

Cat no : 67175-1-Ig

Synonyms

CUL 2, CUL2, cullin 2

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using CUL2 Monoclonal antibody (67175-1-Ig)

    WB analysis of MCF-7 using 67175-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67175-1-Ig (CUL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using CUL2 Monoclonal antibody (67175-1-Ig)

    WB analysis of Raji using 67175-1-Ig

    WB result of CUL2 antibody (67175-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CUL2 transfected Raji cells.

  • Western Blot (WB) analysis of HSC-T6 cells using CUL2 Monoclonal antibody (67175-1-Ig)

    WB analysis of HSC-T6 using 67175-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67175-1-Ig (CUL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using CUL2 Monoclonal antibody (67175-1-Ig)

    WB analysis of PC-3 using 67175-1-Ig

    PC-3 cells were subjected to SDS PAGE followed by western blot with 67175-1-Ig (CUL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CUL2 Monoclonal antibody (67175-1-Ig)

    WB analysis of HeLa using 67175-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67175-1-Ig (CUL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CUL2 Monoclonal antibody (67175-1-Ig)

    WB analysis of HEK-293 using 67175-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67175-1-Ig (CUL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CUL2 Monoclonal antibody (67175-1-Ig)

    IHC staining of human breast cancer using 67175-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67175-1-Ig (CUL2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CUL2 Monoclonal antibody (67175-1-Ig)

    IHC staining of human breast cancer using 67175-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67175-1-Ig (CUL2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CUL2 Monoclonal antibody (67175-1-Ig)

    IHC staining of human prostate cancer using 67175-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67175-1-Ig (CUL2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CUL2 Monoclonal antibody (67175-1-Ig)

    IHC staining of human prostate cancer using 67175-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67175-1-Ig (CUL2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CUL2 Monoclonal antibody (67175-1-Ig)

    IF Staining of HeLa using 67175-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CUL2 antibody (67175-1-Ig, Clone: 1A6G9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells, HEK-293 cells, PC-3 cells, HSC-T6 cells, Raji cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67175-1-Ig targets CUL2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CUL2 fusion protein Ag28654 相同性解析による交差性が予測される生物種
Full Name cullin 2
Calculated molecular weight 87 kDa
Observed molecular weight 85-90 kDa
GenBank accession numberBC009591
Gene symbol CUL2
Gene ID (NCBI) 8453
RRIDAB_2882471
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Humans express seven cullin proteins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.

Protocols

Product Specific Protocols
WB protocol for CUL2 antibody 67175-1-IgDownload protocol
IHC protocol for CUL2 antibody 67175-1-IgDownload protocol
IF protocol for CUL2 antibody 67175-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP,IF

FEBS J

TRAF2 associates with cullin neddylation complex assembly

Authors - Tiantian Wang
View Article