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  • Featured Product
  • KD/KO Validated

CUL2 Monoclonal antibody, PBS Only

CUL2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A6G9

Cat no : 67175-1-PBS

Synonyms

Cullin-2, cullin 2, CUL-2, CUL 2

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using CUL2 Monoclonal antibody (67175-1-Ig)

    WB analysis of MCF-7 using 67175-1-Ig (same clone as 67175-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67175-1-Ig (CUL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CUL2 Monoclonal antibody (67175-1-Ig)

    WB analysis using 67175-1-Ig (same clone as 67175-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67175-1-Ig (CUL2 antibody) at dilution of 1:18000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using CUL2 Monoclonal antibody (67175-1-Ig)

    WB analysis of Raji using 67175-1-Ig (same clone as 67175-1-PBS)

    WB result of CUL2 antibody (67175-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CUL2 transfected Raji cells. This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using CUL2 Monoclonal antibody (67175-1-Ig)

    WB analysis of PC-3 using 67175-1-Ig (same clone as 67175-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 67175-1-Ig (CUL2 antibody) at dilution of 1:18000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SW 1990 cells using CUL2 Monoclonal antibody (67175-1-Ig)

    WB analysis of SW 1990 using 67175-1-Ig (same clone as 67175-1-PBS)

    SW 1990 cells were subjected to SDS PAGE followed by western blot with 67175-1-Ig (CUL2 antibody) at dilution of 1:18000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CUL2 Monoclonal antibody (67175-1-Ig)

    WB analysis of HeLa using 67175-1-Ig (same clone as 67175-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67175-1-Ig (CUL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CUL2 Monoclonal antibody (67175-1-Ig)

    WB analysis of HEK-293 using 67175-1-Ig (same clone as 67175-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67175-1-Ig (CUL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using CUL2 Monoclonal antibody (67175-1-Ig)

    WB analysis of HSC-T6 using 67175-1-Ig (same clone as 67175-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67175-1-Ig (CUL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CUL2 Monoclonal antibody (67175-1-Ig)

    IHC staining of human breast cancer using 67175-1-Ig (same clone as 67175-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67175-1-Ig (CUL2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CUL2 Monoclonal antibody (67175-1-Ig)

    IHC staining of human breast cancer using 67175-1-Ig (same clone as 67175-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67175-1-Ig (CUL2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CUL2 Monoclonal antibody (67175-1-Ig)

    IHC staining of human prostate cancer using 67175-1-Ig (same clone as 67175-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67175-1-Ig (CUL2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CUL2 Monoclonal antibody (67175-1-Ig)

    IHC staining of human prostate cancer using 67175-1-Ig (same clone as 67175-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67175-1-Ig (CUL2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CUL2 Monoclonal antibody (67175-1-Ig)

    IF Staining of HeLa using 67175-1-Ig (same clone as 67175-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CUL2 antibody (67175-1-Ig, Clone: 1A6G9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67175-1-PBS targets CUL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CUL2 fusion protein Ag28654 相同性解析による交差性が予測される生物種
Full Name cullin 2
Calculated molecular weight 87 kDa
Observed molecular weight 85-90 kDa
GenBank accession numberBC009591
Gene symbol CUL2
Gene ID (NCBI) 8453
RRIDAB_2882471
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Humans express seven cullin proteins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.