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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated CUL4A Monoclonal antibody (CL594-66038)

    IF Staining of HepG2 using CL594-66038

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-66038 (CUL4A antibody) at dilution of 1:100.

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  • KD/KO Validated

CoraLite®594-conjugated CUL4A Monoclonal antibody

CUL4A Monoclonal Antibody for IF/ICC
Cat No. CL594-66038
Clone No.1A7F12

Host / Isotype

Mouse / IgG1

Reactivity

human, monkey, mouse, rat, pig

Applications

IF/ICC

Synonyms

CUL 4A, CUL4A, cullin 4A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66038 targets CUL4A in IF/ICC applications and shows reactivity with human, monkey, mouse, rat, pig samples.

Tested Reactivity human, monkey, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CUL4A fusion protein Ag18089 相同性解析による交差性が予測される生物種
Full Name cullin 4A
Calculated molecular weight 77 kDa
Observed molecular weight 77 kDa, 88 kDa
GenBank accession numberBC008308
Gene Symbol CUL4A
Gene ID (NCBI) 8451
RRIDAB_2883486
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13619
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cullin proteins assemble a large number of RING E3 ubiquitin ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), have been identified. The two CUL4 sequences are 83% identical. They target certain proteins for degradation by binding protein DDB1 to form a CUL4-DDB1 ubiquitin ligase complex with DDB. They form two individual E3 ligases, DDB1-CUL4ADDB2 and DDB1-CUL4BDDB2 in this process. CUL4A appeared in both the nucleus and the cytosol, suggesting a more complex mechanism for entering the nucleus. CUL4B is localized in the nucleus and facilitates the transfer of DDB1 into the nucleus independently of DDB2.

Protocols

Product Specific Protocols
IF protocol for CL594 CUL4A antibody CL594-66038Download protocol
Standard Protocols
Click here to view our Standard Protocols