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CoraLite®594-conjugated CUL4A Monoclonal antibody
CUL4A Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
human, monkey, mouse, rat, pig
Applications
IF/ICC
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
1A7F12
Cat no : CL594-66038
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
The immunogen of CL594-66038 is CUL4A Fusion Protein expressed in E. coli.
| Tested Reactivity | human, monkey, mouse, rat, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CUL4A fusion protein Ag18089 相同性解析による交差性が予測される生物種 |
| Full Name | cullin 4A |
| Calculated molecular weight | 77 kDa |
| Observed molecular weight | 77 kDa, 88 kDa |
| GenBank accession number | BC008308 |
| Gene symbol | CUL4A |
| Gene ID (NCBI) | 8451 |
| RRID | AB_2883486 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cullin proteins assemble a large number of RING E3 ubiquitin ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), have been identified. The two CUL4 sequences are 83% identical. They target certain proteins for degradation by binding protein DDB1 to form a CUL4-DDB1 ubiquitin ligase complex with DDB. They form two individual E3 ligases, DDB1-CUL4ADDB2 and DDB1-CUL4BDDB2 in this process. CUL4A appeared in both the nucleus and the cytosol, suggesting a more complex mechanism for entering the nucleus. CUL4B is localized in the nucleus and facilitates the transfer of DDB1 into the nucleus independently of DDB2.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 CUL4A antibody CL594-66038 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
